Detail publikace

In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp.

BREZNA, B. ŠMÍD, J. COSTA, J. RADVANCZKY, J. MAFRA, I. KUCHTA, T.

Originální název

In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp.

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Ten published DNA-based analytical methods aiming at detecting material of almond (Prunus dulcis) were in silica evaluated for potential cross-reactivity with other stone fruits (Prunus spp.), including peach, apricot, plum, cherry, sour cherry and Sargent cherry. For most assays, the analysis of nucleotide databases suggested none or insufficient discrimination of at least some stone fruits. On the other hand, the assay targeting non-specific lipid transfer protein (Roder et al., 2011, Anal Chim Acta 685:74-83) was sufficiently discriminative, judging from nucleotide alignments. Empirical evaluation was performed for three of the published methods, one modification of a commercial kit (SureFood allergen almond) and one attempted novel method targeting thaumatin-like protein gene. Samples of leaves and kernels were used in the experiments. The empirical results were favourable for the method from Roder et al. (2011) and a modification of SureFood allergen almond kit, both showing cross-reactivity <10(-3) compared to the model almond

Klíčová slova

Almond; DNA; Real-time PCR; Food; Allergen

Autoři

BREZNA, B.; ŠMÍD, J.; COSTA, J.; RADVANCZKY, J.; MAFRA, I.; KUCHTA, T.

Vydáno

1. 4. 2015

Nakladatel

Elsevier

ISSN

0890-8508

Periodikum

MOLECULAR AND CELLULAR PROBES

Ročník

29

Číslo

2

Stát

Spojené království Velké Británie a Severního Irska

Strany od

99

Strany do

115

Strany počet

16

URL

https://www.sciencedirect.com/science/article/pii/S0890850814000644

BibTex

@article{BUT163482,
  author="BREZNA, B. and ŠMÍD, J. and COSTA, J. and RADVANCZKY, J. and MAFRA, I. and KUCHTA, T.",
  title="In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp.",
  journal="MOLECULAR AND CELLULAR PROBES",
  year="2015",
  volume="29",
  number="2",
  pages="99--115",
  doi="10.1016/j.mcp.2014.11.006",
  issn="0890-8508",
  url="https://www.sciencedirect.com/science/article/pii/S0890850814000644"
}