Publication detail

The effect of hydrazide linkers on hyaluronan hydrazone hydrogels

ŠEDOVÁ, P. BUFFA, R. ŠILHÁR, P. KOVÁŘOVÁ, L. VÁGNEROVÁ, H. BEDNAŘÍK, J. BASARABOVÁ, I. HEJLOVÁ, L. ŠČIGALKOVÁ, I. ŠIMEK, M. VELEBNÝ, V.

Original Title

The effect of hydrazide linkers on hyaluronan hydrazone hydrogels

Type

journal article in Web of Science

Language

English

Original Abstract

The effect of hydrazide linkers on the formation and mechanical properties of hyaluronan hydrogels was intensively evaluated. The reaction kinetics of hydrazone formation was monitored by NMR spectroscopy under physiological conditions where polyaldehyde hyaluronan (unsaturated: Delta HA-CHO, saturated: HA-CHO) was reacted with various hydrazides to form hydrogels. Linear (adipic, oxalic dihydrazide) and branched (N,N',N ''- tris(hexanoylhydrazide-6-yl)phosphoric triamide and 4-arm-PEG hydrazide) hydrazides were compared as crosslinking agents. The mechanical properties of hydrogels were also modified by attaching a hydrophobic chain to HA-CHO; however, it was found that this modification did not lead to an increase in hydrogel stiffness. Cytotoxicity tests showed that all tested hydrazide crosslinkers reduced the viability of cells only slightly, and that the final hyaluronan hydrogels were non-toxic materials.

Keywords

hyaluronan; hydrogel; hydrazone; Schiff base; acylation

Authors

ŠEDOVÁ, P.; BUFFA, R.; ŠILHÁR, P.; KOVÁŘOVÁ, L.; VÁGNEROVÁ, H.; BEDNAŘÍK, J.; BASARABOVÁ, I.; HEJLOVÁ, L.; ŠČIGALKOVÁ, I.; ŠIMEK, M.; VELEBNÝ, V.

Released

4. 4. 2019

Publisher

ELSEVIER SCI LTD

Location

OXFORD

ISBN

0144-8617

Periodical

Carbohydrate Polymers

Year of study

216

Number

2019

State

United Kingdom of Great Britain and Northern Ireland

Pages from

63

Pages to

71

Pages count

9

URL

https://pubmed.ncbi.nlm.nih.gov/31047083/

BibTex

@article{BUT165168,
  author="ŠEDOVÁ, P. and BUFFA, R. and ŠILHÁR, P. and KOVÁŘOVÁ, L. and VÁGNEROVÁ, H. and BEDNAŘÍK, J. and BASARABOVÁ, I. and HEJLOVÁ, L. and ŠČIGALKOVÁ, I. and ŠIMEK, M. and VELEBNÝ, V.",
  title="The effect of hydrazide linkers on hyaluronan hydrazone hydrogels",
  journal="Carbohydrate Polymers",
  year="2019",
  volume="216",
  number="2019",
  pages="63--71",
  doi="10.1016/j.carbpol.2019.04.011",
  issn="0144-8617",
  url="https://pubmed.ncbi.nlm.nih.gov/31047083/"
}