Publication detail

Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food

FIALOVÁ, L. ROMANOVSKÁ, D. NĚMCOVÁ, A. MÁROVÁ, I.

Original Title

Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food

Type

abstract

Language

English

Original Abstract

Certain species of red fruit, e. g. strawberries, raspberries and blueberries, are expensive components of fruit juices and purees. As a consequence, these products are subject to food fraud. The content of red fruit in them can be lower than declared, or these fruit species can be substituted for cheaper ones. This work explored and expanded a multiplex-PCR based approach to food authentication which has previously been applied to the authentication of buffalo meat products by Sakaridis et al . We successfully used triplex qPCR-HRM with amplicone detection by intercalating dye, and with primers specific for strawberry (Fragaria annanassa), red raspberry (Rubus idaeus) and berries of the Vaccinium genus to prove or disprove the presence of strawberry, red raspberry and bilberry in both model and commercial fruit purees.

Keywords

Multiplex PCR, qPCR-HRM, red fruit, food authentication

Authors

FIALOVÁ, L.; ROMANOVSKÁ, D.; NĚMCOVÁ, A.; MÁROVÁ, I.

Released

30. 10. 2020

ISBN

1314-3530

Periodical

Biotechnology and Biotechnological Equipment

Year of study

35

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

400

Pages to

400

Pages count

424

BibTex

@misc{BUT167775,
  author="Lenka {Fialová} and Denisa {Langová} and Andrea {Němcová} and Ivana {Márová}",
  title="Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food",
  year="2020",
  journal="Biotechnology and Biotechnological Equipment",
  volume="35",
  number="1",
  pages="400--400",
  issn="1314-3530",
  note="abstract"
}