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Detail publikace
FIALOVÁ, L. ROMANOVSKÁ, D. NĚMCOVÁ, A. MÁROVÁ, I.
Originální název
Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food
Typ
abstrakt
Jazyk
angličtina
Originální abstrakt
Certain species of red fruit, e. g. strawberries, raspberries and blueberries, are expensive components of fruit juices and purees. As a consequence, these products are subject to food fraud. The content of red fruit in them can be lower than declared, or these fruit species can be substituted for cheaper ones. This work explored and expanded a multiplex-PCR based approach to food authentication which has previously been applied to the authentication of buffalo meat products by Sakaridis et al . We successfully used triplex qPCR-HRM with amplicone detection by intercalating dye, and with primers specific for strawberry (Fragaria annanassa), red raspberry (Rubus idaeus) and berries of the Vaccinium genus to prove or disprove the presence of strawberry, red raspberry and bilberry in both model and commercial fruit purees.
Klíčová slova
Multiplex PCR, qPCR-HRM, red fruit, food authentication
Autoři
FIALOVÁ, L.; ROMANOVSKÁ, D.; NĚMCOVÁ, A.; MÁROVÁ, I.
Vydáno
30. 10. 2020
ISSN
1314-3530
Periodikum
Biotechnology and Biotechnological Equipment
Ročník
35
Číslo
1
Stát
Spojené království Velké Británie a Severního Irska
Strany od
400
Strany do
Strany počet
424
BibTex
@misc{BUT167775, author="Lenka {Fialová} and Denisa {Langová} and Andrea {Němcová} and Ivana {Márová}", title="Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food", year="2020", journal="Biotechnology and Biotechnological Equipment", volume="35", number="1", pages="400--400", issn="1314-3530", note="abstract" }