Product detail

EnzymeMiner: automated mining of soluble enzymes with diverse structures, catalytic properties and stabilities

BORKO, S. HON, J. BEDNÁŘ, D. DAMBORSKÝ, J. MARTÍNEK, T. PROKOP, Z. ŠTOURAČ, J. ZENDULKA, J.

Product type

software

Abstract

EnzymeMiner prioritizes sequences that are more likely to preserve the catalytic activity and are heterologously expressible in a soluble form in Escherichia coli. The solubility prediction employs the in-house SoluProt predictor developed using machine learning. EnzymeMiner reduces the time devoted to data gathering, multi-step analysis, sequence prioritization and selection from days to hours. The successful use case for the haloalkane dehalogenase family is described in a comprehensive tutorial available on the EnzymeMiner web page. EnzymeMiner is a universal tool applicable to any enzyme family that provides an interactive and easy-to-use web interface freely available at https://loschmidt.chemi.muni.cz/enzymeminer/.

Keywords

computational characterization, enzyme mining, enzyme diversity, novel biocatalysts

Create date

2. 6. 2020

Location

https://loschmidt.chemi.muni.cz/enzymeminer/

Possibilities of use

Využití výsledku jiným subjektem je v některých případech možné bez nabytí licence

Licence fee

Poskytovatel licence na výsledek nepožaduje licenční poplatek

www

https://loschmidt.chemi.muni.cz/enzymeminer/