Publication detail

CalFitter: a web server for analysis of protein thermal denaturation data

DAMBORSKÝ, J. BEDNÁŘ, D. KUNKA, A. MAZURENKO, S. NEDELJKOVIĆ, S. PROKOP, Z. ŠTOURAČ, J.

Original Title

CalFitter: a web server for analysis of protein thermal denaturation data

Type

journal article in Web of Science

Language

English

Original Abstract

Despite significant advances in the understanding of protein structure-function relationships, revealing protein folding pathways still poses a challenge due to a limited number of relevant experimental tools. Widely-used experimental techniques, such as calorimetry or spectroscopy, critically depend on a proper data analysis. Currently, there are only separate data analysis tools available for each type of experiment with a limited model selection. To address this problem, we have developed the CalFitter web server to be a unified platform for comprehensive data fitting and analysis of protein thermal denaturation data. The server allows simultaneous global data fitting using any combination of input data types and offers twelve protein unfolding pathway models for selection, including irreversible transitions often missing from other tools. The data fitting produces optimal parameters, their confidence intervals, and statistical information to define unfolding pathways. The server provides an interactive and easy-to-use interface that allows users to directly analyse input datasets and simulate modelled output based on the model parameters. CalFitter web server is available free at https://loschmidt.chemi.muni.cz/calfitter/.

Keywords

Computational Bilology, Protein Denaturation, Internet, Protein Unfolding, Software

Authors

DAMBORSKÝ, J.; BEDNÁŘ, D.; KUNKA, A.; MAZURENKO, S.; NEDELJKOVIĆ, S.; PROKOP, Z.; ŠTOURAČ, J.

Released

2. 7. 2018

ISBN

1362-4962

Periodical

Nucleic Acids Research

Year of study

46

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

344

Pages to

349

Pages count

6

URL

https://academic.oup.com/nar/article/46/W1/W344/4995690

BibTex

@article{BUT170435,
  author="Jiří {Damborský} and David {Bednář} and Antonín {Kunka} and Stanislav {Mazurenko} and Sava {Nedeljković} and Zbyněk {Prokop} and Jan {Štourač}",
  title="CalFitter: a web server for analysis of protein thermal denaturation data",
  journal="Nucleic Acids Research",
  year="2018",
  volume="46",
  number="1",
  pages="344--349",
  doi="10.1093/nar/gky358",
  issn="1362-4962",
  url="https://academic.oup.com/nar/article/46/W1/W344/4995690"
}