Detail publikace

CalFitter: a web server for analysis of protein thermal denaturation data

DAMBORSKÝ, J. BEDNÁŘ, D. KUNKA, A. MAZURENKO, S. NEDELJKOVIĆ, S. PROKOP, Z. ŠTOURAČ, J.

Originální název

CalFitter: a web server for analysis of protein thermal denaturation data

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Despite significant advances in the understanding of protein structure-function relationships, revealing protein folding pathways still poses a challenge due to a limited number of relevant experimental tools. Widely-used experimental techniques, such as calorimetry or spectroscopy, critically depend on a proper data analysis. Currently, there are only separate data analysis tools available for each type of experiment with a limited model selection. To address this problem, we have developed the CalFitter web server to be a unified platform for comprehensive data fitting and analysis of protein thermal denaturation data. The server allows simultaneous global data fitting using any combination of input data types and offers twelve protein unfolding pathway models for selection, including irreversible transitions often missing from other tools. The data fitting produces optimal parameters, their confidence intervals, and statistical information to define unfolding pathways. The server provides an interactive and easy-to-use interface that allows users to directly analyse input datasets and simulate modelled output based on the model parameters. CalFitter web server is available free at https://loschmidt.chemi.muni.cz/calfitter/.

Klíčová slova

Computational Bilology, Protein Denaturation, Internet, Protein Unfolding, Software

Autoři

DAMBORSKÝ, J.; BEDNÁŘ, D.; KUNKA, A.; MAZURENKO, S.; NEDELJKOVIĆ, S.; PROKOP, Z.; ŠTOURAČ, J.

Vydáno

2. 7. 2018

ISSN

1362-4962

Periodikum

Nucleic Acids Research

Ročník

46

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

344

Strany do

349

Strany počet

6

URL

https://academic.oup.com/nar/article/46/W1/W344/4995690

BibTex

@article{BUT170435,
  author="Jiří {Damborský} and David {Bednář} and Antonín {Kunka} and Stanislav {Mazurenko} and Sava {Nedeljković} and Zbyněk {Prokop} and Jan {Štourač}",
  title="CalFitter: a web server for analysis of protein thermal denaturation data",
  journal="Nucleic Acids Research",
  year="2018",
  volume="46",
  number="1",
  pages="344--349",
  doi="10.1093/nar/gky358",
  issn="1362-4962",
  url="https://academic.oup.com/nar/article/46/W1/W344/4995690"
}