Publication detail

Using QD-FRET based method to investigate protein-protein interactions

PAVELICOVÁ, K. ZEMÁNKOVÁ, K. BEZDĚKOVÁ, J. VACULOVIČOVÁ, M.

Original Title

Using QD-FRET based method to investigate protein-protein interactions

Type

conference paper

Language

English

Original Abstract

Förster resonance energy transfer (FRET) is an energy transfer process between a pair of light-sensitive molecules, where the donor fluorophore (initially in its electronic excited state) transfers energy to an acceptor chromophore. In this study, FRET was used to investigate the dimerization of metallothionein isoform MT-1A. The FRET system was developed based on a fluorescent quantum dots (QDs) and cyanine dye 3.5 (Cy3.5) as a versatile tool to probe small distance changes between acceptor and donor fluorophores in nanometer range. Herein, the water-soluble 450-nm emitting QDs as the donor and 590-nm Cy3.5 as the acceptor were covalently conjugated with MT-1 according to the protocol. Previous studies suggest that MTs may form oligomers under certain conditions. Therefore, further studies of this phenomenon, which has been studied here using capillary electrophoresis (CE) coupled with fluorescence detection.

Keywords

Förster resonance energy transfer (FRET), quantum dots (QDs), Oligomerization, Capillary electrophoresis, Metallothionein

Authors

PAVELICOVÁ, K.; ZEMÁNKOVÁ, K.; BEZDĚKOVÁ, J.; VACULOVIČOVÁ, M.

Released

12. 7. 2021

Publisher

Tanger

ISBN

978-80-87294-98-7

Book

NANOCON 2020 CONFERENCE PROCEEDINGS

Edition

1st

ISBN

2694-930X

Periodical

Proceedings 12th International Conference on Nanomaterials - Research & Application

State

Czech Republic

Pages from

390

Pages to

394

Pages count

5

URL

https://www.confer.cz/nanocon/2020/3750-using-qd-fret-based-method-to-investigate-protein-protein-interactions

Full text in the Digital Library

http://hdl.handle.net/11012/200498

BibTex

@inproceedings{BUT172013,
  author="Kristýna {Pavelicová} and Kristýna {Zemánková} and Jaroslava {Bezděková} and Markéta {Vaculovičová}",
  title="Using QD-FRET based method to investigate protein-protein interactions",
  booktitle="NANOCON 2020 CONFERENCE PROCEEDINGS",
  year="2021",
  series="1st",
  journal="Proceedings 12th International Conference on Nanomaterials - Research & Application",
  pages="390--394",
  publisher="Tanger",
  doi="10.37904/nanocon.2020.3750",
  isbn="978-80-87294-98-7",
  issn="2694-930X",
  url="https://www.confer.cz/nanocon/2020/3750-using-qd-fret-based-method-to-investigate-protein-protein-interactions"
}