Detail publikace

Using QD-FRET based method to investigate protein-protein interactions

PAVELICOVÁ, K. ZEMÁNKOVÁ, K. BEZDĚKOVÁ, J. VACULOVIČOVÁ, M.

Originální název

Using QD-FRET based method to investigate protein-protein interactions

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Förster resonance energy transfer (FRET) is an energy transfer process between a pair of light-sensitive molecules, where the donor fluorophore (initially in its electronic excited state) transfers energy to an acceptor chromophore. In this study, FRET was used to investigate the dimerization of metallothionein isoform MT-1A. The FRET system was developed based on a fluorescent quantum dots (QDs) and cyanine dye 3.5 (Cy3.5) as a versatile tool to probe small distance changes between acceptor and donor fluorophores in nanometer range. Herein, the water-soluble 450-nm emitting QDs as the donor and 590-nm Cy3.5 as the acceptor were covalently conjugated with MT-1 according to the protocol. Previous studies suggest that MTs may form oligomers under certain conditions. Therefore, further studies of this phenomenon, which has been studied here using capillary electrophoresis (CE) coupled with fluorescence detection.

Klíčová slova

Förster resonance energy transfer (FRET), quantum dots (QDs), Oligomerization, Capillary electrophoresis, Metallothionein

Autoři

PAVELICOVÁ, K.; ZEMÁNKOVÁ, K.; BEZDĚKOVÁ, J.; VACULOVIČOVÁ, M.

Vydáno

12. 7. 2021

Nakladatel

Tanger

ISBN

978-80-87294-98-7

Kniha

NANOCON 2020 CONFERENCE PROCEEDINGS

Edice

1st

ISSN

2694-930X

Periodikum

Proceedings 12th International Conference on Nanomaterials - Research & Application

Stát

Česká republika

Strany od

390

Strany do

394

Strany počet

5

URL

https://www.confer.cz/nanocon/2020/3750-using-qd-fret-based-method-to-investigate-protein-protein-interactions

Plný text v Digitální knihovně

http://hdl.handle.net/11012/200498

BibTex

@inproceedings{BUT172013,
  author="Kristýna {Pavelicová} and Kristýna {Zemánková} and Jaroslava {Bezděková} and Markéta {Vaculovičová}",
  title="Using QD-FRET based method to investigate protein-protein interactions",
  booktitle="NANOCON 2020 CONFERENCE PROCEEDINGS",
  year="2021",
  series="1st",
  journal="Proceedings 12th International Conference on Nanomaterials - Research & Application",
  pages="390--394",
  publisher="Tanger",
  doi="10.37904/nanocon.2020.3750",
  isbn="978-80-87294-98-7",
  issn="2694-930X",
  url="https://www.confer.cz/nanocon/2020/3750-using-qd-fret-based-method-to-investigate-protein-protein-interactions"
}