Publication detail

In Situ Investigation of the Cytotoxic and Interfacial Characteristics of Titanium When Galvanically Coupled with Magnesium Using Scanning Electrochemical Microscopy

ASSERGHINE, A. ASHRAFI, A. MUKHERJEE, A. PETRLÁK, F. HEGER, Z. ŠVEC, P. RICHTERA, L. NAGY, L. SOUTO, R. NAGY, G. ADAM, V.

Original Title

In Situ Investigation of the Cytotoxic and Interfacial Characteristics of Titanium When Galvanically Coupled with Magnesium Using Scanning Electrochemical Microscopy

Type

journal article in Web of Science

Language

English

Original Abstract

Recently, the cytotoxic properties of galvanically coupled Ti-Mg particles have been shown in different cells. This cytotoxic effect has been attributed mainly to Mg due to its tendency to undergo activation when coupled with Ti, forming a galvanic cell consisting of an anode (Mg) and a cathode (Ti). However, the role of the Ti cathode has been ignored in explaining the cytotoxic effect of Ti-Mg particles due to its high resistance to corrosion. In this work, the role of titanium (Ti) in the cytotoxic mechanism of galvanically coupled Ti-Mg particles was examined. A model galvanic cell (MGC) was prepared to simulate the Mg-Ti particles. The electrochemical reactivity of the Ti sample and the pH change in it due to galvanic coupling with Mg were investigated using scanning electrochemical microscopy (SECM). It was observed that the Ti surface changed from passive to electrochemically active when coupled with Mg. Furthermore, after only 15 min of galvanic coupling with Mg, the pH in the electrolyte volume adjacent to the Ti surface increased to an alkaline pH value. The effects of the galvanic coupling of Ti and Mg, as well as those of the alkaline pH environment, on the viability of Hs27 fibroblast cells were investigated. It was shown that the viability of Hs27 cells significantly diminished when Mg and Ti were galvanically coupled compared to when the two metals were electrically disconnected. Thus, although Ti usually exhibited high corrosion resistance when exposed to physiological environments, an electrochemically active surface was observed when galvanically coupled with Mg, and this surface may participate in electron transfer reactions with chemical species in the neighboring environment; this participation resulted in the increased pH values above its surface and enhanced generation of reactive oxygen species. These features contributed to the development of cytotoxic effects by galvanically coupled Ti-Mg particles.

Keywords

titanium; magnesium; galvanic coupling; local electrochemical activity; cytotoxic effects; pH variations; reactive oxygen species

Authors

ASSERGHINE, A.; ASHRAFI, A.; MUKHERJEE, A.; PETRLÁK, F.; HEGER, Z.; ŠVEC, P.; RICHTERA, L.; NAGY, L.; SOUTO, R.; NAGY, G.; ADAM, V.

Released

15. 9. 2021

ISBN

1944-8244

Periodical

ACS APPL MATER INTER

Year of study

13

Number

36

State

United States of America

Pages from

43587

Pages to

43596

Pages count

10

URL

https://pubs.acs.org/doi/abs/10.1021/acsami.1c10584

BibTex

@article{BUT172671,
  author="ASSERGHINE, A. and ASHRAFI, A. and MUKHERJEE, A. and PETRLÁK, F. and HEGER, Z. and ŠVEC, P. and RICHTERA, L. and NAGY, L. and SOUTO, R. and NAGY, G. and ADAM, V.",
  title="In Situ Investigation of the Cytotoxic and Interfacial Characteristics of Titanium When Galvanically Coupled with Magnesium Using Scanning Electrochemical Microscopy",
  journal="ACS APPL MATER INTER",
  year="2021",
  volume="13",
  number="36",
  pages="43587--43596",
  doi="10.1021/acsami.1c10584",
  issn="1944-8244",
  url="https://pubs.acs.org/doi/abs/10.1021/acsami.1c10584"
}