Publication detail

UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors

KHUNKITCHAI, N. NUCHTAVORN, N. RÝPAR, T. VLČNOVSKÁ, M. NEJDL, L. VACULOVIČOVÁ, M. MACKA, M.

Original Title

UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors

Type

journal article in Web of Science

Language

English

Original Abstract

Quantum dots (QDs) have been widely applied in the analytical field including sensitive fluorescent assays on paper-based devices. A facile in-situ synthesis of QDs after a simple reagent deposition on paper could enable a rapid low-cost fabrication of QD–modified paper with a variety of properties and purposes such as enzymatic nanoreactors. Herein, for the first time, fluorescent ZnCd QDs were prepared by in-situ synthesis in the paper matrix using thiol-containing precursors and irradiation by UV light. A successful creation of the immobilized ZnCd QDs on paper (paper@ZnCd QDs) was monitored by their intrinsic fluorescence while their peroxidase mimetic activity was evaluated by a catalytic reaction between H2O2 and a substrate (3,3′,5,5′-tetramethylbenzidine, TMB) producing a blue coloured product of charged transfer complex of TMB (oxTMB). From three thiol precursors investigated, the insulin precursor provided a greater activity than glutathione (GSH) and both considerably larger than BSA. Finally, a QDs precursor mixture deposition onto the paper matrix was evaluated by the peroxidase-mimetic activity, which was comparable with a reference method. The in-situ preparation of paper@ZnCd QDs is simple, rapid and ‘green’, with potential in biomedical applications primarily as fluorescence imaging agent and enzyme mimetic paper-based nanoreactors.

Keywords

Fluorescent quantum dots; In-situ synthesis; Microfluidic paper-based devices; Paperfluidics; Enzyme mimetics; Nanoreactor

Authors

KHUNKITCHAI, N.; NUCHTAVORN, N.; RÝPAR, T.; VLČNOVSKÁ, M.; NEJDL, L.; VACULOVIČOVÁ, M.; MACKA, M.

Released

15. 1. 2022

ISBN

1385-8947

Periodical

CHEMICAL ENGINEERING JOURNAL

Year of study

428

Number

132508

State

Swiss Confederation

Pages from

1

Pages to

5

Pages count

5

URL

https://doi.org/10.1016/j.cej.2021.132508

BibTex

@article{BUT176902,
  author="KHUNKITCHAI, N. and NUCHTAVORN, N. and RÝPAR, T. and VLČNOVSKÁ, M. and NEJDL, L. and VACULOVIČOVÁ, M. and MACKA, M.",
  title="UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors",
  journal="CHEMICAL ENGINEERING JOURNAL",
  year="2022",
  volume="428",
  number="132508",
  pages="1--5",
  doi="10.1016/j.cej.2021.132508",
  issn="1385-8947",
  url="https://doi.org/10.1016/j.cej.2021.132508"
}