Detail publikace

UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors

KHUNKITCHAI, N. NUCHTAVORN, N. RÝPAR, T. VLČNOVSKÁ, M. NEJDL, L. VACULOVIČOVÁ, M. MACKA, M.

Originální název

UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Quantum dots (QDs) have been widely applied in the analytical field including sensitive fluorescent assays on paper-based devices. A facile in-situ synthesis of QDs after a simple reagent deposition on paper could enable a rapid low-cost fabrication of QD–modified paper with a variety of properties and purposes such as enzymatic nanoreactors. Herein, for the first time, fluorescent ZnCd QDs were prepared by in-situ synthesis in the paper matrix using thiol-containing precursors and irradiation by UV light. A successful creation of the immobilized ZnCd QDs on paper (paper@ZnCd QDs) was monitored by their intrinsic fluorescence while their peroxidase mimetic activity was evaluated by a catalytic reaction between H2O2 and a substrate (3,3′,5,5′-tetramethylbenzidine, TMB) producing a blue coloured product of charged transfer complex of TMB (oxTMB). From three thiol precursors investigated, the insulin precursor provided a greater activity than glutathione (GSH) and both considerably larger than BSA. Finally, a QDs precursor mixture deposition onto the paper matrix was evaluated by the peroxidase-mimetic activity, which was comparable with a reference method. The in-situ preparation of paper@ZnCd QDs is simple, rapid and ‘green’, with potential in biomedical applications primarily as fluorescence imaging agent and enzyme mimetic paper-based nanoreactors.

Klíčová slova

Fluorescent quantum dots; In-situ synthesis; Microfluidic paper-based devices; Paperfluidics; Enzyme mimetics; Nanoreactor

Autoři

KHUNKITCHAI, N.; NUCHTAVORN, N.; RÝPAR, T.; VLČNOVSKÁ, M.; NEJDL, L.; VACULOVIČOVÁ, M.; MACKA, M.

Vydáno

15. 1. 2022

ISSN

1385-8947

Periodikum

CHEMICAL ENGINEERING JOURNAL

Ročník

428

Číslo

132508

Stát

Švýcarská konfederace

Strany od

1

Strany do

5

Strany počet

5

URL

https://doi.org/10.1016/j.cej.2021.132508

BibTex

@article{BUT176902,
  author="KHUNKITCHAI, N. and NUCHTAVORN, N. and RÝPAR, T. and VLČNOVSKÁ, M. and NEJDL, L. and VACULOVIČOVÁ, M. and MACKA, M.",
  title="UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors",
  journal="CHEMICAL ENGINEERING JOURNAL",
  year="2022",
  volume="428",
  number="132508",
  pages="1--5",
  doi="10.1016/j.cej.2021.132508",
  issn="1385-8947",
  url="https://doi.org/10.1016/j.cej.2021.132508"
}