Publication detail

qPCR identification of selected probiotic bacteria in fer-mented white cabbage juicemented white cabbage juic

STREČANSKÁ, P. HOOVÁ, J. SKOUMALOVÁ, P. MÁROVÁ, I.

Original Title

qPCR identification of selected probiotic bacteria in fer-mented white cabbage juicemented white cabbage juic

Type

journal article - other

Language

English

Original Abstract

The juice made from fermented cabbage is a traditional drink frequently consumed as a source of probiotic bacteria. We in-vestigated cabbage juice as a natural substrate for the growth of probiotic cultures before and after pasteurisation using molecular methods. According to the literature, four natu-rally occurring probiotic organisms in cabbage juice were selected (L. acidophilus, L. casei, L. delbruckii subsp. bul-garicus, and L. plantarum), and their occurrence was subsequently monitored in the samples using real-time PCR. The PCR products were detected on an agarose gel. Firstly, DNA amplification was verified using specific prim-ers for the genus Lactobacillus and individual species of pro-biotic microorganisms. The genus Lactobacillus’s presence was confirmed in fresh and pasteurized cabbage juice. Due to the decrease of the probiotic viability after pasteurization, the cabbage juice was enriched with the addition of probiot-ics. Their presence was monitored using cultivation methods and qPCR for two weeks after inoculation. The presence of individual selected species in conditions of enriched cab-bage juice was confirmed for all samples in both time inter-vals, although with a low percentage of probiotic viability.

Keywords

cabbage juice, probiotics, qPCR, primers, amplification

Authors

STREČANSKÁ, P.; HOOVÁ, J.; SKOUMALOVÁ, P.; MÁROVÁ, I.

Released

1. 2. 2023

Publisher

Sciendo

Location

Italy

ISBN

2564-615X

Periodical

The EuroBiotech Journal

Year of study

7

Number

1

State

Republic of Italy

Pages from

45

Pages to

46

Pages count

2

URL

https://sciendo.com/article/10.2478/ebtj-2023-0004

BibTex

@article{BUT182975,
  author="Paulína {Strečanská} and Julie {Hoová} and Petra {Skoumalová} and Ivana {Márová}",
  title="qPCR identification of selected probiotic bacteria in fer-mented white cabbage juicemented white cabbage juic",
  journal="The EuroBiotech Journal",
  year="2023",
  volume="7",
  number="1",
  pages="45--46",
  doi="10.2478/ebtj-2023-0004",
  issn="2564-615X",
  url="https://sciendo.com/article/10.2478/ebtj-2023-0004"
}