Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
STREČANSKÁ, P. HOOVÁ, J. SKOUMALOVÁ, P. MÁROVÁ, I.
Originální název
qPCR identification of selected probiotic bacteria in fer-mented white cabbage juicemented white cabbage juic
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
The juice made from fermented cabbage is a traditional drink frequently consumed as a source of probiotic bacteria. We in-vestigated cabbage juice as a natural substrate for the growth of probiotic cultures before and after pasteurisation using molecular methods. According to the literature, four natu-rally occurring probiotic organisms in cabbage juice were selected (L. acidophilus, L. casei, L. delbruckii subsp. bul-garicus, and L. plantarum), and their occurrence was subsequently monitored in the samples using real-time PCR. The PCR products were detected on an agarose gel. Firstly, DNA amplification was verified using specific prim-ers for the genus Lactobacillus and individual species of pro-biotic microorganisms. The genus Lactobacillus’s presence was confirmed in fresh and pasteurized cabbage juice. Due to the decrease of the probiotic viability after pasteurization, the cabbage juice was enriched with the addition of probiot-ics. Their presence was monitored using cultivation methods and qPCR for two weeks after inoculation. The presence of individual selected species in conditions of enriched cab-bage juice was confirmed for all samples in both time inter-vals, although with a low percentage of probiotic viability.
Klíčová slova
cabbage juice, probiotics, qPCR, primers, amplification
Autoři
STREČANSKÁ, P.; HOOVÁ, J.; SKOUMALOVÁ, P.; MÁROVÁ, I.
Vydáno
1. 2. 2023
Nakladatel
Sciendo
Místo
Italy
ISSN
2564-615X
Periodikum
The EuroBiotech Journal
Ročník
7
Číslo
1
Stát
Italská republika
Strany od
45
Strany do
46
Strany počet
2
URL
https://sciendo.com/article/10.2478/ebtj-2023-0004
BibTex
@article{BUT182975, author="Paulína {Strečanská} and Julie {Hoová} and Petra {Skoumalová} and Ivana {Márová}", title="qPCR identification of selected probiotic bacteria in fer-mented white cabbage juicemented white cabbage juic", journal="The EuroBiotech Journal", year="2023", volume="7", number="1", pages="45--46", doi="10.2478/ebtj-2023-0004", issn="2564-615X", url="https://sciendo.com/article/10.2478/ebtj-2023-0004" }