Publication detail

Enhancing Wound Healing with Bioprinted Chitosan Hydrogels Treated by Cold Atmospheric Plasma Jets

MOHAMAD, N. EZATI, M. HASHEMI, A. POLÁŠKOVÁ, K. ZUMBERG, I. ČMIEL, V. SCHÄFER, J. ZAJÍČKOVÁ, L.

Original Title

Enhancing Wound Healing with Bioprinted Chitosan Hydrogels Treated by Cold Atmospheric Plasma Jets

Type

abstract

Language

English

Original Abstract

Uncontrolled bleeding in many kinds of injuries and accidents is a major factor leading to death. Severe blood loss can result in hypothermia, coagulopathy, and multiorgan failure, while bacterial infections can further complicate the recovery process. Addressing this, we investigate the application of plasma treatment to improve the antibacterial characteristics of chitosan (CS) hydrogels and augment their suitability for bioprinting applications. We employ 3D bioprinting to fabricate CS hydrogel scaffolds because it has the capacity to closely mimic the architecture of human tissues. The 3D printing precision allows fabrication of specific supports and improved transfer of essential nutrients, which is crucial in cases of severe bleeding where conventional hydrogels and dressings may not be adequate. Plasma activation enhances hydrogels by introducing oxygen functionalities, including reactive oxygen and nitrogen species (RONS), providing a novel approach to wound healing. We investigated two approaches that use cold atmospheric pressure plasma jets: plasma treatment of uncrosslinked CS solutions (A) and surface modification of printed CS hydrogel scaffolds (B). Our initial findings suggested that the plasma-treated hydrogel scaffolds exhibit higher hydrophilicity, i. e. reduction of water contact angle. Cell viability experiments showed that plasma-treated hydrogels significantly enhanced fibroblast cell morphology and growth. Our findings indicate that plasma-treated CS hydrogels may find applications in wound dressings.

Keywords

Plasma, RONS, Chitosan, Hydrogel, Bioprinting, Wound healing

Authors

MOHAMAD, N.; EZATI, M.; HASHEMI, A.; POLÁŠKOVÁ, K.; ZUMBERG, I.; ČMIEL, V.; SCHÄFER, J.; ZAJÍČKOVÁ, L.

Released

13. 9. 2024

Publisher

Ljubljana, Jožef Stefan Institute

Location

Ljubljana, Slovenia

ISBN

978-961-264-293-8

Book

Book of abstracts – 10th International Conference on Plasma Medicine and 9th International Workshop on Plasma for Cancer Treatment

Pages from

207

Pages to

207

Pages count

1

URL

https://f6-ge.ijs.si/icpm10/?page_id=1269

BibTex

@misc{BUT188672,
  author="Noor {Mohamad} and Masoumeh {Ezati} and Amir {Hashemi} and Kateřina {Polášková} and Inna {Zumberg} and Vratislav {Čmiel} and Jan {Schäfer} and Lenka {Zajíčková}",
  title="Enhancing Wound Healing with Bioprinted Chitosan Hydrogels Treated by Cold Atmospheric Plasma Jets",
  booktitle="Book of abstracts – 10th International Conference on Plasma Medicine and 9th International Workshop on Plasma for Cancer Treatment",
  year="2024",
  pages="207--207",
  publisher="Ljubljana, Jožef Stefan Institute",
  address="Ljubljana, Slovenia",
  isbn="978-961-264-293-8",
  url="https://f6-ge.ijs.si/icpm10/?page_id=1269",
  note="abstract"
}