Publication detail

Horizontal transmission of a multidrug-resistant IncN plasmid isolated from urban wastewater

Yu, ZF. Wang, QQ. Pinilla-Redondo, R. Madsen, JS. Clasen, KAD. Ananbeh, H. Olesen, AK. Gong, Z. Yang, N. Dechesne, A. Smets, B. Nesme, J. Sorensen, SJ.

Original Title

Horizontal transmission of a multidrug-resistant IncN plasmid isolated from urban wastewater

Type

journal article in Web of Science

Language

English

Original Abstract

Wastewater treatment plants (WWTPs) are considered reservoirs of antibiotic resistance genes (ARGs). Given that plasmid-mediated horizontal gene transfer plays a critical role in disseminating ARGs in the environment, it is important to inspect the transfer potential of transmissible plasmids to have a better understanding of whether these mobile ARGs can be hosted by opportunistic pathogens and should be included in One Health's considerations. In this study, we used a fluorescent-reporter-gene based exogenous isolation approach to capture extended-spectrum beta-lactamases encoding mobile determinants from sewer microbiome samples that enter an urban water system (UWS) in Denmark. After screening and sequencing, we isolated a similar to 73 Kbp IncN plasmid (pDK_DARWIN) that harboured and expressed multiple ARGs. Using a dual fluorescent reporter gene system, we showed that this plasmid can transfer into resident urban water communities. We demonstrated the transfer of pDK_DARWIN to microbiome members of both the sewer (in the upstream UWS compartment) and wastewater treatment (in the downstream UWS compartment) microbiomes. Sequence similarity search across curated plasmid repositories revealed that pDK_DARWIN derives from an IncN backbone harboured by environmental and nosocomial Enterobacterial isolates. Furthermore, we searched for pDK_DARWIN sequence matches in UWS metagenomes from three countries, revealing that this plasmid can be detected in all of them, with a higher relative abundance in hospital sewers compared to residential sewers. Overall, this study demonstrates that this IncN plasmid is prevalent across Europe and an efficient vector capable of disseminating multiple ARGs in the urban water systems.

Keywords

IncN; Plasmids; Antibiotic Resistance; Horizontal Gene Transfer; Urban Wastewater

Authors

Yu, ZF.; Wang, QQ.; Pinilla-Redondo, R.; Madsen, JS.; Clasen, KAD.; Ananbeh, H.; Olesen, AK.; Gong, Z.; Yang, N.; Dechesne, A.; Smets, B.; Nesme, J.; Sorensen, SJ.

Released

1. 2. 2024

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE

Location

SAN DIEGO

ISBN

1090-2414

Periodical

Ecotoxicology and Environmental Safety

Year of study

271

Number

115971

State

United Kingdom of Great Britain and Northern Ireland

Pages count

12

URL

https://www.sciencedirect.com/science/article/pii/S0147651324000460?via%3Dihub

BibTex

@article{BUT188946,
  author="Yu, ZF. and Wang, QQ. and Pinilla-Redondo, R. and Madsen, JS. and Clasen, KAD. and Ananbeh, H. and Olesen, AK. and Gong, Z. and Yang, N. and Dechesne, A. and Smets, B. and Nesme, J. and Sorensen, SJ.",
  title="Horizontal transmission of a multidrug-resistant IncN plasmid isolated from urban wastewater",
  journal="Ecotoxicology and Environmental Safety",
  year="2024",
  volume="271",
  number="115971",
  pages="12",
  doi="10.1016/j.ecoenv.2024.115971",
  issn="1090-2414",
  url="https://www.sciencedirect.com/science/article/pii/S0147651324000460?via%3Dihub"
}