Detail publikace

Horizontal transmission of a multidrug-resistant IncN plasmid isolated from urban wastewater

Yu, ZF. Wang, QQ. Pinilla-Redondo, R. Madsen, JS. Clasen, KAD. Ananbeh, H. Olesen, AK. Gong, Z. Yang, N. Dechesne, A. Smets, B. Nesme, J. Sorensen, SJ.

Originální název

Horizontal transmission of a multidrug-resistant IncN plasmid isolated from urban wastewater

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Wastewater treatment plants (WWTPs) are considered reservoirs of antibiotic resistance genes (ARGs). Given that plasmid-mediated horizontal gene transfer plays a critical role in disseminating ARGs in the environment, it is important to inspect the transfer potential of transmissible plasmids to have a better understanding of whether these mobile ARGs can be hosted by opportunistic pathogens and should be included in One Health's considerations. In this study, we used a fluorescent-reporter-gene based exogenous isolation approach to capture extended-spectrum beta-lactamases encoding mobile determinants from sewer microbiome samples that enter an urban water system (UWS) in Denmark. After screening and sequencing, we isolated a similar to 73 Kbp IncN plasmid (pDK_DARWIN) that harboured and expressed multiple ARGs. Using a dual fluorescent reporter gene system, we showed that this plasmid can transfer into resident urban water communities. We demonstrated the transfer of pDK_DARWIN to microbiome members of both the sewer (in the upstream UWS compartment) and wastewater treatment (in the downstream UWS compartment) microbiomes. Sequence similarity search across curated plasmid repositories revealed that pDK_DARWIN derives from an IncN backbone harboured by environmental and nosocomial Enterobacterial isolates. Furthermore, we searched for pDK_DARWIN sequence matches in UWS metagenomes from three countries, revealing that this plasmid can be detected in all of them, with a higher relative abundance in hospital sewers compared to residential sewers. Overall, this study demonstrates that this IncN plasmid is prevalent across Europe and an efficient vector capable of disseminating multiple ARGs in the urban water systems.

Klíčová slova

IncN; Plasmids; Antibiotic Resistance; Horizontal Gene Transfer; Urban Wastewater

Autoři

Yu, ZF.; Wang, QQ.; Pinilla-Redondo, R.; Madsen, JS.; Clasen, KAD.; Ananbeh, H.; Olesen, AK.; Gong, Z.; Yang, N.; Dechesne, A.; Smets, B.; Nesme, J.; Sorensen, SJ.

Vydáno

1. 2. 2024

Nakladatel

ACADEMIC PRESS INC ELSEVIER SCIENCE

Místo

SAN DIEGO

ISSN

1090-2414

Periodikum

Ecotoxicology and Environmental Safety

Ročník

271

Číslo

115971

Stát

Spojené království Velké Británie a Severního Irska

Strany počet

12

URL

https://www.sciencedirect.com/science/article/pii/S0147651324000460?via%3Dihub

BibTex

@article{BUT188946,
  author="Yu, ZF. and Wang, QQ. and Pinilla-Redondo, R. and Madsen, JS. and Clasen, KAD. and Ananbeh, H. and Olesen, AK. and Gong, Z. and Yang, N. and Dechesne, A. and Smets, B. and Nesme, J. and Sorensen, SJ.",
  title="Horizontal transmission of a multidrug-resistant IncN plasmid isolated from urban wastewater",
  journal="Ecotoxicology and Environmental Safety",
  year="2024",
  volume="271",
  number="115971",
  pages="12",
  doi="10.1016/j.ecoenv.2024.115971",
  issn="1090-2414",
  url="https://www.sciencedirect.com/science/article/pii/S0147651324000460?via%3Dihub"
}