Publication detail

Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa

Lipovy, B. Vacek, L. Kleknerova, DP. Jeklova, E. Liskova, L. Holoubek, J. Matyskova, D. Ruzicka, F

Original Title

Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa

Type

journal article in Web of Science

Language

English

Original Abstract

Pseudomonas aeruginosa poses a significant threat to both immunocompetent and immunocompromised individuals, often resulting in life -threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for P. aeruginosa complicated skin and soft tissue infections (cSSTIs). This study presents a novel porcine model inducing and sustaining cSSTI for 14 days. Six pigs (120 wounds) were used for the development of infections, and within this group, two pigs (40 wounds) were used to evaluate the progression of the cSSTI infection. The model demonstrated bacterial loads of more than 10 7 CFU/ gram of tissue or higher. The cSSTI fully developed within three days and remained well above these levels until day 14 post -infection. Due to the immunocompetence of this model, all the immunological processes associated with the response to the presence of infection and the wound healing process are preserved.

Keywords

ESKAPE pathogen; model development; pig; wound

Authors

Lipovy, B.; Vacek, L.; Kleknerova, DP.; Jeklova, E.; Liskova, L.; Holoubek, J.; Matyskova, D.; Ruzicka, F

Released

10. 9. 2024

Publisher

CZECH ACADEMY AGRICULTURAL SCIENCES

Location

PRAGUE

ISBN

1805-9392

Periodical

VETERINARNI MEDICINA

Year of study

69

Number

6

State

Czech Republic

Pages from

191

Pages to

197

Pages count

7

URL

https://vetmed.agriculturejournals.cz/artkey/vet-202406-0004_porcine-model-of-a-complicated-skin-and-soft-tissue-infection-caused-by-pseudomonas-aeruginosa.php

BibTex

@article{BUT189940,
  author="Břetislav {Lipový} and Lukáš {Vacek} and Dominika {Polaštík Kleknerová} and Edita {Jeklová} and Lenka {Lišková} and Jakub {Holoubek} and Dominika {Matýsková} and Filip {Růžička}",
  title="Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa",
  journal="VETERINARNI MEDICINA",
  year="2024",
  volume="69",
  number="6",
  pages="191--197",
  doi="10.17221/25/2024-VETMED",
  issn="1805-9392",
  url="https://vetmed.agriculturejournals.cz/artkey/vet-202406-0004_porcine-model-of-a-complicated-skin-and-soft-tissue-infection-caused-by-pseudomonas-aeruginosa.php"
}