Publication detail

In Vivo Assessment on Freeze-Cast Calcium Phosphate-Based Scaffolds with a Selective Cell/Tissue Ingrowth

Pejchalová, L. Pejchal, J. Roleček, J. Vojníkova, M. Chlup, Z. Mařák, V. González-Sánchez, M. Čížková, J. Salamon, D.

Original Title

In Vivo Assessment on Freeze-Cast Calcium Phosphate-Based Scaffolds with a Selective Cell/Tissue Ingrowth

Type

journal article in Web of Science

Language

English

Original Abstract

Highly porous bioceramic scaffolds are widely used as bone substitutes in many applications. However, the use of bioceramics is often limited to hard tissues due to the risk of potential soft tissue calcification. A further limitation of highly porous bioceramic scaffolds is their poor mechanical stability, manifested by their tendency to break under stress. In our study, highly porous CaP-based scaffolds were prepared via freeze-casting with longitudinal and oriented pores ranging from 10 to 20 mu m and a relative porosity of similar to 70%. The resulting scaffolds achieved a flexural strength of 10.6 +/- 2.7 MPa, which, in conjunction with their favorable bioactivity, made them suitable for in vivo testing. The prepared scaffolds were subcutaneously implanted in rats for two distinct periods: 6 weeks and 6 months, respectively. The subsequent development of fibrous tissue and involvement of myofibroblasts, newly formed vessels, and macrophages were observed, with notable changes in spatial and temporal distributions within the implantation. The absence of calcification in the surrounding soft tissue, as a result of the narrow pore geometry, indicates the opportunity to tailor the scaffold behavior for soft tissue regeneration.

Keywords

in vivo; tissue engineering; scaffolds; calcium phosphates; freeze-casting; bioceramics

Authors

Pejchalová, L.; Pejchal, J.; Roleček, J.; Vojníkova, M.; Chlup, Z.; Mařák, V.; González-Sánchez, M.; Čížková, J.; Salamon, D.

Released

21. 10. 2024

Publisher

AMER CHEMICAL SOC

Location

WASHINGTON

ISBN

1944-8244

Periodical

ACS APPL MATER INTER

Year of study

16

Number

43

State

United States of America

Pages from

58326

Pages to

58336

Pages count

11

URL

https://pubs.acs.org/doi/10.1021/acsami.4c12715

Full text in the Digital Library

http://hdl.handle.net/11012/250794

BibTex

@article{BUT191213,
  author="Lucie {Mařáková} and Jaroslav {Pejchal} and Jakub {Roleček} and Michaela {Vojníková} and Zdeněk {Chlup} and Vojtěch {Mařák} and Manuela {González-Sánchez} and Jana {Čížková} and David {Salamon}",
  title="In Vivo Assessment on Freeze-Cast Calcium Phosphate-Based Scaffolds with a Selective Cell/Tissue Ingrowth",
  journal="ACS APPL MATER INTER",
  year="2024",
  volume="16",
  number="43",
  pages="58326--58336",
  doi="10.1021/acsami.4c12715",
  issn="1944-8244",
  url="https://pubs.acs.org/doi/10.1021/acsami.4c12715"
}