Publication detail

Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device

BEZDĚKOVÁ, J. PLEVOVÁ, M. NEJDL, L. MACKA, M. MASAŘÍK, M. PACÍK, D. ADAM, V. VACULOVIČOVÁ, M.

Original Title

Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device

Type

journal article in Web of Science

Language

English

Original Abstract

The early detection of prostate cancer (CaP), one of the most common cancers in males, improves treatment efficacy. However, current methods to detect CaP are limited by specificity and sensitivity or require an invasive and unpleasant procedure. Therefore, there is a need for a novel, fast, and noninvasive CaP detection method. This proof-of-concept study aimed to diagnose CaP from urine samples by detecting the UV-induced fluorescent clusters formed in situ that reflect a urine composition specific to CaP patients using fluorescence spectroscopy. The UV-induced clusters formed upon irradiation by UV light with a wavelength of 254 nm were detected at excitation and emission wavelengths of 400 nm and 460 nm. Fifteen patients with CaP were correctly distinguished from 5 controls. The different stages of CaP could be further determined using molecular imprinting technology. Additionally, a paper-based analytical device was developed that enabled the correct identification of stage I cancer patients by fluorescent spectroscopy detection and the naked eye. In conclusion, this novel, easyto-operate, point-of-care assay can enable early diagnosis of CaP with high accuracy and judgement of the disease stage. The simplicity, as well as versatility of the proposed method, will enable its application also to other diseases and disorders.

Keywords

Molecularly imprinted polymers; UV -induced fluorescent clusters; UV -induced fluorescence fingerprinting

Authors

BEZDĚKOVÁ, J.; PLEVOVÁ, M.; NEJDL, L.; MACKA, M.; MASAŘÍK, M.; PACÍK, D.; ADAM, V.; VACULOVIČOVÁ, M.

Released

15. 3. 2024

Publisher

ELSEVIER SCIENCE SA

Location

LAUSANNE

ISBN

0925-4005

Periodical

Sensors and Actuators B: Chemical

Year of study

403

Number

135146

State

Swiss Confederation

Pages count

10

URL

https://www.sciencedirect.com/science/article/pii/S0925400523018646?via%3Dihub

BibTex

@article{BUT197281,
  author="Jaroslava {Bezděková} and Mariana {Plevová} and Lukáš {Nejdl} and Michal {Masařík} and Dalibor {Pacík} and Vojtěch {Adam} and Markéta {Vaculovičová} and Miroslav {Macka}",
  title="Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device",
  journal="Sensors and Actuators B: Chemical",
  year="2024",
  volume="403",
  number="135146",
  pages="10",
  doi="10.1016/j.snb.2023.135146",
  issn="0925-4005",
  url="https://www.sciencedirect.com/science/article/pii/S0925400523018646?via%3Dihub"
}