Detail publikace

Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device

BEZDĚKOVÁ, J. PLEVOVÁ, M. NEJDL, L. MACKA, M. MASAŘÍK, M. PACÍK, D. ADAM, V. VACULOVIČOVÁ, M.

Originální název

Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The early detection of prostate cancer (CaP), one of the most common cancers in males, improves treatment efficacy. However, current methods to detect CaP are limited by specificity and sensitivity or require an invasive and unpleasant procedure. Therefore, there is a need for a novel, fast, and noninvasive CaP detection method. This proof-of-concept study aimed to diagnose CaP from urine samples by detecting the UV-induced fluorescent clusters formed in situ that reflect a urine composition specific to CaP patients using fluorescence spectroscopy. The UV-induced clusters formed upon irradiation by UV light with a wavelength of 254 nm were detected at excitation and emission wavelengths of 400 nm and 460 nm. Fifteen patients with CaP were correctly distinguished from 5 controls. The different stages of CaP could be further determined using molecular imprinting technology. Additionally, a paper-based analytical device was developed that enabled the correct identification of stage I cancer patients by fluorescent spectroscopy detection and the naked eye. In conclusion, this novel, easyto-operate, point-of-care assay can enable early diagnosis of CaP with high accuracy and judgement of the disease stage. The simplicity, as well as versatility of the proposed method, will enable its application also to other diseases and disorders.

Klíčová slova

Molecularly imprinted polymers; UV -induced fluorescent clusters; UV -induced fluorescence fingerprinting

Autoři

BEZDĚKOVÁ, J.; PLEVOVÁ, M.; NEJDL, L.; MACKA, M.; MASAŘÍK, M.; PACÍK, D.; ADAM, V.; VACULOVIČOVÁ, M.

Vydáno

15. 3. 2024

Nakladatel

ELSEVIER SCIENCE SA

Místo

LAUSANNE

ISSN

0925-4005

Periodikum

Sensors and Actuators B: Chemical

Ročník

403

Číslo

135146

Stát

Švýcarská konfederace

Strany počet

10

URL

BibTex

@article{BUT197281,
  author="Jaroslava {Bezděková} and Mariana {Plevová} and Lukáš {Nejdl} and Michal {Masařík} and Dalibor {Pacík} and Vojtěch {Adam} and Markéta {Vaculovičová} and Miroslav {Macka}",
  title="Prostate cancer diagnosed and staged using UV-irradiated urine samples and a paper-based analytical device",
  journal="Sensors and Actuators B: Chemical",
  year="2024",
  volume="403",
  number="135146",
  pages="10",
  doi="10.1016/j.snb.2023.135146",
  issn="0925-4005",
  url="https://www.sciencedirect.com/science/article/pii/S0925400523018646?via%3Dihub"
}