Publication detail

Capillary isoelectric focusing with UV-induced fluorescence detection

Horká, M., Willimann, T., Blum, M., Nording, P., Friedl, Z., Šlais, K.

Original Title

Capillary isoelectric focusing with UV-induced fluorescence detection

Type

journal article - other

Language

English

Original Abstract

Low-molecular fluorescent compounds excitable in the near UV region with suitable acidobasic and electrophoretic properties suggested as isoelectric point (pI) markers for electric focusing (IEF) with UV photometric and UV excited fluorimetric detection. The experimental set-up of capillary IEF with UV excited fluorometric detection and properties of new UV-induced fluorescent pI markers are given. The pI values of 18 new pI markers determined independently of IEF methods range from 2.1 to 10.3. The examples of separation of new pI markers together with derivatized proteins by capillary IEF with photometric detection are presented.

Key words in English

isolecric focusing, detection, electrophoresis, fluorescence detection, proteins

Authors

Horká, M., Willimann, T., Blum, M., Nording, P., Friedl, Z., Šlais, K.

RIV year

2000

Released

31. 1. 2001

ISBN

0021-9673

Periodical

Journal of Chromatography A

Year of study

916

Number

1

State

Kingdom of the Netherlands

Pages from

65

Pages to

71

Pages count

7

BibTex

@article{BUT39416,
  author="Marie {Horká} and T. {Willimann} and M. {Blum} and Pierre {Nording} and Zdeněk {Friedl} and Karel {Šlais}",
  title="Capillary isoelectric focusing with UV-induced fluorescence detection",
  journal="Journal of Chromatography A",
  year="2001",
  volume="916",
  number="1",
  pages="7",
  issn="0021-9673"
}