Detail publikace

Capillary isoelectric focusing with UV-induced fluorescence detection

Horká, M., Willimann, T., Blum, M., Nording, P., Friedl, Z., Šlais, K.

Originální název

Capillary isoelectric focusing with UV-induced fluorescence detection

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

Low-molecular fluorescent compounds excitable in the near UV region with suitable acidobasic and electrophoretic properties suggested as isoelectric point (pI) markers for electric focusing (IEF) with UV photometric and UV excited fluorimetric detection. The experimental set-up of capillary IEF with UV excited fluorometric detection and properties of new UV-induced fluorescent pI markers are given. The pI values of 18 new pI markers determined independently of IEF methods range from 2.1 to 10.3. The examples of separation of new pI markers together with derivatized proteins by capillary IEF with photometric detection are presented.

Klíčová slova v angličtině

isolecric focusing, detection, electrophoresis, fluorescence detection, proteins

Autoři

Horká, M., Willimann, T., Blum, M., Nording, P., Friedl, Z., Šlais, K.

Rok RIV

2000

Vydáno

31. 1. 2001

ISSN

0021-9673

Periodikum

Journal of Chromatography A

Ročník

916

Číslo

1

Stát

Nizozemsko

Strany od

65

Strany do

71

Strany počet

7

BibTex

@article{BUT39416,
  author="Marie {Horká} and T. {Willimann} and M. {Blum} and Pierre {Nording} and Zdeněk {Friedl} and Karel {Šlais}",
  title="Capillary isoelectric focusing with UV-induced fluorescence detection",
  journal="Journal of Chromatography A",
  year="2001",
  volume="916",
  number="1",
  pages="7",
  issn="0021-9673"
}