Publication detail

Employment of Electrochemical Techniques for Metallothionein Determination in Tumor Cell Lines and Patients with a Tumor Disease

FABRIK, I. KŘÍŽKOVÁ, S. HÚSKA, D. ADAM, V. HUBÁLEK, J. TRNKOVÁ, L. PRŮŠA, R. KIZEK, R.

Original Title

Employment of Electrochemical Techniques for Metallothionein Determination in Tumor Cell Lines and Patients with a Tumor Disease

Type

journal article - other

Language

English

Original Abstract

In the present paper we employed adsorptive transfer stripping technique coupled with chronopotentiometric stripping analysis for determination of metallothionein (MT) in tumor cell lines and differential pulse voltammetry Brdicka reaction for determination of MT in blood serum of patients with head and neck cancer or retinoblastoma, and of rats treated with cisplatin with respect to discuss the role of MT in formation of resistance on treatment with heavy metal based cytostatics. The cisplatin or carboplatin sensitive and resistant neuroblastoma cell lines were derived from the maternal cell line isolated from the bone metastasis of patients with neuroblastoma. Based on the results obtained it can be concluded that level of MT increases with higher dose of platinum based cytostatics at cells. Further we focused on determination of MT in blood serum of rats treated with cisplatin (two doses 1.05 mg and/or 2.1 mg of cisplatin per kg). The highest level of MT at rats treated with 1.05 mg cisplatin was determined after four hours as 4.9 mmol/L.

Keywords

Metallothionein, Carboplatin, Cisplatin, Neuroblastoma cell, Retinoblastoma, Head and neck cancer, Resistance, Voltammetry, Brdicka reaction

Authors

FABRIK, I.; KŘÍŽKOVÁ, S.; HÚSKA, D.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; PRŮŠA, R.; KIZEK, R.

RIV year

2008

Released

15. 5. 2008

Publisher

WILEY-VCH Verlag GmbH&Co.

Location

Weinheim

ISBN

1040-0397

Periodical

ELECTROANALYSIS

Year of study

20

Number

14

State

Federal Republic of Germany

Pages from

1521

Pages to

1532

Pages count

11

BibTex

@article{BUT47208,
  author="Ivo {Fabrik} and Soňa {Křížková} and Dalibor {Húska} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and Richard {Průša} and René {Kizek}",
  title="Employment of Electrochemical Techniques for Metallothionein Determination in Tumor Cell Lines and Patients with a Tumor Disease",
  journal="ELECTROANALYSIS",
  year="2008",
  volume="20",
  number="14",
  pages="1521--1532",
  issn="1040-0397"
}