Detail publikace

Employment of Electrochemical Techniques for Metallothionein Determination in Tumor Cell Lines and Patients with a Tumor Disease

FABRIK, I. KŘÍŽKOVÁ, S. HÚSKA, D. ADAM, V. HUBÁLEK, J. TRNKOVÁ, L. PRŮŠA, R. KIZEK, R.

Originální název

Employment of Electrochemical Techniques for Metallothionein Determination in Tumor Cell Lines and Patients with a Tumor Disease

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

In the present paper we employed adsorptive transfer stripping technique coupled with chronopotentiometric stripping analysis for determination of metallothionein (MT) in tumor cell lines and differential pulse voltammetry Brdicka reaction for determination of MT in blood serum of patients with head and neck cancer or retinoblastoma, and of rats treated with cisplatin with respect to discuss the role of MT in formation of resistance on treatment with heavy metal based cytostatics. The cisplatin or carboplatin sensitive and resistant neuroblastoma cell lines were derived from the maternal cell line isolated from the bone metastasis of patients with neuroblastoma. Based on the results obtained it can be concluded that level of MT increases with higher dose of platinum based cytostatics at cells. Further we focused on determination of MT in blood serum of rats treated with cisplatin (two doses 1.05 mg and/or 2.1 mg of cisplatin per kg). The highest level of MT at rats treated with 1.05 mg cisplatin was determined after four hours as 4.9 mmol/L.

Klíčová slova

Metallothionein, Carboplatin, Cisplatin, Neuroblastoma cell, Retinoblastoma, Head and neck cancer, Resistance, Voltammetry, Brdicka reaction

Autoři

FABRIK, I.; KŘÍŽKOVÁ, S.; HÚSKA, D.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; PRŮŠA, R.; KIZEK, R.

Rok RIV

2008

Vydáno

15. 5. 2008

Nakladatel

WILEY-VCH Verlag GmbH&Co.

Místo

Weinheim

ISSN

1040-0397

Periodikum

ELECTROANALYSIS

Ročník

20

Číslo

14

Stát

Spolková republika Německo

Strany od

1521

Strany do

1532

Strany počet

11

BibTex

@article{BUT47208,
  author="Ivo {Fabrik} and Soňa {Křížková} and Dalibor {Húska} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and Richard {Průša} and René {Kizek}",
  title="Employment of Electrochemical Techniques for Metallothionein Determination in Tumor Cell Lines and Patients with a Tumor Disease",
  journal="ELECTROANALYSIS",
  year="2008",
  volume="20",
  number="14",
  pages="1521--1532",
  issn="1040-0397"
}