Publication detail

Optimalization of Cultivation Media and Hydroxylase Enzyme Production by Aureobasidium Pullulans

OMELKOVÁ, J.

Original Title

Optimalization of Cultivation Media and Hydroxylase Enzyme Production by Aureobasidium Pullulans

Type

journal article - other

Language

English

Original Abstract

The present paper aims to study the influence of medium composition, temperature, method of inoculation and process of cultivation on growth of Aureobasidium pullulans. In the current work being conducted , cross-linked wheat straw were used as substrate. Aureobasidium pullulans was tested for the ability to produce hydroxylases, especially laccase and also for the ability to produce release - reducing sugars and proteins in basal media. Laccase oxidized a redox indicator ABTS ( 2,2' -azino-di (3-ethylbenzothiazoline- 6-sulfonic acid ) to form a blue - green cation radical.The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates. The method can be adapted for quantitative estimation by spectrophotometry. Aureobasidium pullulans were grown in different type of solid media, but less growth was observed in the course of liquid cultivations regardless of temperature. During the optimalization of the culture medium hydroxylase activity reached higher level in the presence of yeast Aureobasidium pulllulans in the glucose medium without wheat straw during submerged cultivation.

Keywords

Keywords: Aureobasidium pullulans, wheat straw

Authors

OMELKOVÁ, J.

RIV year

2008

Released

9. 9. 2008

ISBN

1213-7103

Periodical

Chemické listy

Year of study

102

Number

15

State

Czech Republic

Pages from

745

Pages to

746

Pages count

2

BibTex

@article{BUT47268,
  author="Jiřina {Omelková}",
  title="Optimalization of Cultivation Media and Hydroxylase Enzyme Production by Aureobasidium Pullulans",
  journal="Chemické listy",
  year="2008",
  volume="102",
  number="15",
  pages="745--746",
  issn="1213-7103"
}