Detail publikace

Optimalization of Cultivation Media and Hydroxylase Enzyme Production by Aureobasidium Pullulans

OMELKOVÁ, J.

Originální název

Optimalization of Cultivation Media and Hydroxylase Enzyme Production by Aureobasidium Pullulans

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

The present paper aims to study the influence of medium composition, temperature, method of inoculation and process of cultivation on growth of Aureobasidium pullulans. In the current work being conducted , cross-linked wheat straw were used as substrate. Aureobasidium pullulans was tested for the ability to produce hydroxylases, especially laccase and also for the ability to produce release - reducing sugars and proteins in basal media. Laccase oxidized a redox indicator ABTS ( 2,2' -azino-di (3-ethylbenzothiazoline- 6-sulfonic acid ) to form a blue - green cation radical.The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates. The method can be adapted for quantitative estimation by spectrophotometry. Aureobasidium pullulans were grown in different type of solid media, but less growth was observed in the course of liquid cultivations regardless of temperature. During the optimalization of the culture medium hydroxylase activity reached higher level in the presence of yeast Aureobasidium pulllulans in the glucose medium without wheat straw during submerged cultivation.

Klíčová slova

Keywords: Aureobasidium pullulans, wheat straw

Autoři

OMELKOVÁ, J.

Rok RIV

2008

Vydáno

9. 9. 2008

ISSN

1213-7103

Periodikum

Chemické listy

Ročník

102

Číslo

15

Stát

Česká republika

Strany od

745

Strany do

746

Strany počet

2

BibTex

@article{BUT47268,
  author="Jiřina {Omelková}",
  title="Optimalization of Cultivation Media and Hydroxylase Enzyme Production by Aureobasidium Pullulans",
  journal="Chemické listy",
  year="2008",
  volume="102",
  number="15",
  pages="745--746",
  issn="1213-7103"
}