Publication detail

Metallomics of melanoma animal tissues

MASAŘÍK, Michal - ZÍTKA, Ondřej - HÚSKA, Dalibor - KŘÍŽKOVÁ, Soňa - STRNADEL, Ján - HORÁK, Vratislav - VACULOVIČ, Tomáš - NOVOTNÝ, Karel - KANICKÝ, Viktor - KAISER, Josef - ADAM, Vojtěch - KIZEK, René

Original Title

Metallomics of melanoma animal tissues

Type

abstract

Language

English

Original Abstract

Metallomics and metalloproteomics are emerging fields addressing the role, uptake, transport and storage of trace metals essential for life. There are several main approaches that are being developed in metallomics and metalloproteomics including both detection of levels and spatial distribution of heavy metals and determination of heavy-metal-contained proteins. The aim of this work is detection of copper and zinc in healthy and tumour tissues of miniature pigs by using of the laser induced breakdown spectroscopy (LIBS). Concentration of heavy metal transporting protein metallothionein (MT) was determined by Brdicka reaction. Tissue cryosections were obtained from the MeLiM strain of miniature pigs with hereditary melanoma, particularly from healthy skin, cutaneous nodular melanomas and metastases in the liver, spleen and lymph nodes. Using LIBS we measured maps of spatial distribution of the essential heavy metals in cryosections and found that the maps of healthy and tumour cryosection markedly differed. The highest content of MT was determined in the tumours localised on the back of animals and was nearly 500 ug of MT per gram of tissue.

Keywords

metallomics; LIBS; electrochemistry; melanoma

Authors

MASAŘÍK, Michal - ZÍTKA, Ondřej - HÚSKA, Dalibor - KŘÍŽKOVÁ, Soňa - STRNADEL, Ján - HORÁK, Vratislav - VACULOVIČ, Tomáš - NOVOTNÝ, Karel - KANICKÝ, Viktor - KAISER, Josef - ADAM, Vojtěch - KIZEK, René

RIV year

2009

Released

1. 12. 2009

ISBN

1107-3756

Periodical

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Year of study

24

Number

Suppl. 1

State

United States of America

Pages from

S49

Pages to

S49

Pages count

1