Detail publikace

Metallomics of melanoma animal tissues

MASAŘÍK, Michal - ZÍTKA, Ondřej - HÚSKA, Dalibor - KŘÍŽKOVÁ, Soňa - STRNADEL, Ján - HORÁK, Vratislav - VACULOVIČ, Tomáš - NOVOTNÝ, Karel - KANICKÝ, Viktor - KAISER, Josef - ADAM, Vojtěch - KIZEK, René

Originální název

Metallomics of melanoma animal tissues

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Metallomics and metalloproteomics are emerging fields addressing the role, uptake, transport and storage of trace metals essential for life. There are several main approaches that are being developed in metallomics and metalloproteomics including both detection of levels and spatial distribution of heavy metals and determination of heavy-metal-contained proteins. The aim of this work is detection of copper and zinc in healthy and tumour tissues of miniature pigs by using of the laser induced breakdown spectroscopy (LIBS). Concentration of heavy metal transporting protein metallothionein (MT) was determined by Brdicka reaction. Tissue cryosections were obtained from the MeLiM strain of miniature pigs with hereditary melanoma, particularly from healthy skin, cutaneous nodular melanomas and metastases in the liver, spleen and lymph nodes. Using LIBS we measured maps of spatial distribution of the essential heavy metals in cryosections and found that the maps of healthy and tumour cryosection markedly differed. The highest content of MT was determined in the tumours localised on the back of animals and was nearly 500 ug of MT per gram of tissue.

Klíčová slova

metallomics; LIBS; electrochemistry; melanoma

Autoři

MASAŘÍK, Michal - ZÍTKA, Ondřej - HÚSKA, Dalibor - KŘÍŽKOVÁ, Soňa - STRNADEL, Ján - HORÁK, Vratislav - VACULOVIČ, Tomáš - NOVOTNÝ, Karel - KANICKÝ, Viktor - KAISER, Josef - ADAM, Vojtěch - KIZEK, René

Rok RIV

2009

Vydáno

1. 12. 2009

ISSN

1107-3756

Periodikum

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Ročník

24

Číslo

Suppl. 1

Stát

Spojené státy americké

Strany od

S49

Strany do

S49

Strany počet

1