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HÚSKA, D. FABRIK, I. BALOUN, J. ADAM, V. MASAŘÍK, M. HUBÁLEK, J. VAŠKŮ, A. TRNKOVÁ, L. HORNA, A. ZEMAN, L. KIZEK, R.
Original Title
Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance
Type
journal article - other
Language
English
Original Abstract
Treatment strategies for tumour diseases are progressively focusing on personalization of medicine. However, this focus requires methods revealing the early general biological mechanisms, including the formation anti-cancer drugs' resistance. The low molecular mass protein metallothionein is thought to be the crucial for the formation of resistance in tumour treatment based on the platinum-cytostatics. The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdicka's reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.
Keywords
Cancer; Metallothionein; Cisplatin; Protein-Drug Interaction; Voltammetry; Brdicka's reaction; Quartz Crystal Microbalance
Authors
HÚSKA, D.; FABRIK, I.; BALOUN, J.; ADAM, V.; MASAŘÍK, M.; HUBÁLEK, J.; VAŠKŮ, A.; TRNKOVÁ, L.; HORNA, A.; ZEMAN, L.; KIZEK, R.
RIV year
2009
Released
15. 3. 2009
Publisher
MDPI
Location
MATTHAEUSSTRASSE 11, CH-4057 BASEL, SWITZERLAND
ISBN
1424-8220
Periodical
SENSORS
Year of study
2009 (9)
Number
3
State
Swiss Confederation
Pages from
1355
Pages to
1369
Pages count
15
URL
http://www.mdpi.com/1424-8220/9/3/1355
Full text in the Digital Library
http://hdl.handle.net/11012/70083
BibTex
@article{BUT48743, author="HÚSKA, D. and FABRIK, I. and BALOUN, J. and ADAM, V. and MASAŘÍK, M. and HUBÁLEK, J. and VAŠKŮ, A. and TRNKOVÁ, L. and HORNA, A. and ZEMAN, L. and KIZEK, R.", title="Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance", journal="SENSORS", year="2009", volume="2009 (9)", number="3", pages="1355--1369", doi="10.3390/s90301355", issn="1424-8220", url="http://www.mdpi.com/1424-8220/9/3/1355" }