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HÚSKA, D. FABRIK, I. BALOUN, J. ADAM, V. MASAŘÍK, M. HUBÁLEK, J. VAŠKŮ, A. TRNKOVÁ, L. HORNA, A. ZEMAN, L. KIZEK, R.
Originální název
Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
Treatment strategies for tumour diseases are progressively focusing on personalization of medicine. However, this focus requires methods revealing the early general biological mechanisms, including the formation anti-cancer drugs' resistance. The low molecular mass protein metallothionein is thought to be the crucial for the formation of resistance in tumour treatment based on the platinum-cytostatics. The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdicka's reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.
Klíčová slova
Cancer; Metallothionein; Cisplatin; Protein-Drug Interaction; Voltammetry; Brdicka's reaction; Quartz Crystal Microbalance
Autoři
HÚSKA, D.; FABRIK, I.; BALOUN, J.; ADAM, V.; MASAŘÍK, M.; HUBÁLEK, J.; VAŠKŮ, A.; TRNKOVÁ, L.; HORNA, A.; ZEMAN, L.; KIZEK, R.
Rok RIV
2009
Vydáno
15. 3. 2009
Nakladatel
MDPI
Místo
MATTHAEUSSTRASSE 11, CH-4057 BASEL, SWITZERLAND
ISSN
1424-8220
Periodikum
SENSORS
Ročník
2009 (9)
Číslo
3
Stát
Švýcarská konfederace
Strany od
1355
Strany do
1369
Strany počet
15
URL
http://www.mdpi.com/1424-8220/9/3/1355
Plný text v Digitální knihovně
http://hdl.handle.net/11012/70083
BibTex
@article{BUT48743, author="HÚSKA, D. and FABRIK, I. and BALOUN, J. and ADAM, V. and MASAŘÍK, M. and HUBÁLEK, J. and VAŠKŮ, A. and TRNKOVÁ, L. and HORNA, A. and ZEMAN, L. and KIZEK, R.", title="Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance", journal="SENSORS", year="2009", volume="2009 (9)", number="3", pages="1355--1369", doi="10.3390/s90301355", issn="1424-8220", url="http://www.mdpi.com/1424-8220/9/3/1355" }