Přístupnostní navigace
E-application
Search Search Close
Publication detail
JANEČKOVÁ, H. VESELÝ, P. CHMELÍK, R.
Original Title
Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy
Type
journal article in Web of Science
Language
English
Original Abstract
Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by an acute nutritional and energy deprivation as a survival threat. A phosphate-buffered saline (PBS) survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hours. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal cells. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed an increase in the compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. Studying the PBS survival test with DHM opens a new approach to investigations of the structural integrity of neoplastic cells.
Keywords
Tumour cell, nutritional deprivation, energy deprivation, cell survival, cell death, digital holographic microscopy, dynamic phase difference
Authors
JANEČKOVÁ, H.; VESELÝ, P.; CHMELÍK, R.
RIV year
2009
Released
6. 6. 2009
Publisher
International Institute of Anticancer Research
Location
Greece
ISBN
0250-7005
Periodical
ANTICANCER RESEARCH
Year of study
29
Number
6
State
Hellenic Republic
Pages from
2339
Pages to
2345
Pages count
7
URL
http://www.iiar-anticancer.org/
BibTex
@article{BUT49714, author="Hana {Uhlířová} and Pavel {Veselý} and Radim {Chmelík}", title="Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy", journal="ANTICANCER RESEARCH", year="2009", volume="29", number="6", pages="2339--2345", issn="0250-7005", url="http://www.iiar-anticancer.org/" }