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JANEČKOVÁ, H. VESELÝ, P. CHMELÍK, R.
Originální název
Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by an acute nutritional and energy deprivation as a survival threat. A phosphate-buffered saline (PBS) survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hours. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal cells. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed an increase in the compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. Studying the PBS survival test with DHM opens a new approach to investigations of the structural integrity of neoplastic cells.
Klíčová slova
Tumour cell, nutritional deprivation, energy deprivation, cell survival, cell death, digital holographic microscopy, dynamic phase difference
Autoři
JANEČKOVÁ, H.; VESELÝ, P.; CHMELÍK, R.
Rok RIV
2009
Vydáno
6. 6. 2009
Nakladatel
International Institute of Anticancer Research
Místo
Greece
ISSN
0250-7005
Periodikum
ANTICANCER RESEARCH
Ročník
29
Číslo
6
Stát
Řecká republika
Strany od
2339
Strany do
2345
Strany počet
7
URL
http://www.iiar-anticancer.org/
BibTex
@article{BUT49714, author="Hana {Uhlířová} and Pavel {Veselý} and Radim {Chmelík}", title="Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy", journal="ANTICANCER RESEARCH", year="2009", volume="29", number="6", pages="2339--2345", issn="0250-7005", url="http://www.iiar-anticancer.org/" }