Publication detail

The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation

ČUTA, R. FRIČOVÁ, M. ŠPANOVÁ, A. RITTICH, B.

Original Title

The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation

Type

abstract

Language

English

Original Abstract

The preparation of crude lysates of bacterial cells is relatively expensive procedure because of the enzymes are used during the procedure. This problem can be solved by using of the washing powders for the cell lysis. The aim of our work was the isolation of DNA from the Lactobacillus at quality for the PCRs and to test whether the DNA isolated by the magnetic microsperes (P(HEMA-co-GMA) is appropriate to run the PCR with. We tried to find out if the composition of the washing powders is suitable for disruption of the cell wall instead of lysosyme.

Keywords

crude lyzates, Lactobacillus, isolation, DNA, PCR

Authors

ČUTA, R.; FRIČOVÁ, M.; ŠPANOVÁ, A.; RITTICH, B.

Released

17. 8. 2011

Publisher

Česká společnost chemická

Location

Praha

ISBN

0009-2770

Periodical

Chemické listy

Year of study

105

Number

18

State

Czech Republic

Pages from

s1009

Pages to

s1009

Pages count

1