Detail publikace

The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation

ČUTA, R. FRIČOVÁ, M. ŠPANOVÁ, A. RITTICH, B.

Originální název

The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

The preparation of crude lysates of bacterial cells is relatively expensive procedure because of the enzymes are used during the procedure. This problem can be solved by using of the washing powders for the cell lysis. The aim of our work was the isolation of DNA from the Lactobacillus at quality for the PCRs and to test whether the DNA isolated by the magnetic microsperes (P(HEMA-co-GMA) is appropriate to run the PCR with. We tried to find out if the composition of the washing powders is suitable for disruption of the cell wall instead of lysosyme.

Klíčová slova

crude lyzates, Lactobacillus, isolation, DNA, PCR

Autoři

ČUTA, R.; FRIČOVÁ, M.; ŠPANOVÁ, A.; RITTICH, B.

Vydáno

17. 8. 2011

Nakladatel

Česká společnost chemická

Místo

Praha

ISSN

0009-2770

Periodikum

Chemické listy

Ročník

105

Číslo

18

Stát

Česká republika

Strany od

s1009

Strany do

s1009

Strany počet

1