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FRIČOVÁ, M. ČUTA, R. ŠPANOVÁ, A. RITTICH, B.
Original Title
Alternative procedure for preparation of Lactobacillus cell lysates
Type
abstract
Language
English
Original Abstract
The Lactobacillus species play an important role in the food industry.They are also very important for human health due to their positive influence of the gastrointestinal tract functions. Identification of the Lactobacillus species is based on the DNA amplification methods. Molecular methods are essential for correct identification of Lactobacillus at the genus or species levels. Polymerase chain reaction (PCR) is common amplification method used in biological laboratories. PCR-ready DNA must be available to amplification. Enzymes and detergents are used for the crude cell lysates preparation. Enzymes disrupt the cell wall and cell proteins and detergents destroy the plasmatic membrane of the cells. DNA is relased into medium and it is available for purification procedure. Lysozyme, sodium dodecyl sulphate (SDS) and proteinase K are the standard components in the bacterial crude lysates preparations. They are relativelly expensive and sophisticated to storage. The aim of this work was find out if enzymes and detergents presented in the washing powders are capable of cell lysis at adequate quality.
Keywords
crude lysates, Lactobacillus, proteinase K, SDS, DNA
Authors
FRIČOVÁ, M.; ČUTA, R.; ŠPANOVÁ, A. ; RITTICH, B.
Released
17. 8. 2011
Publisher
Česká společnost chemická
Location
Praha
ISBN
0009-2770
Periodical
Chemické listy
Year of study
105
Number
18
State
Czech Republic
Pages from
s1011
Pages to
Pages count
1