Detail publikace

Alternative procedure for preparation of Lactobacillus cell lysates

FRIČOVÁ, M. ČUTA, R. ŠPANOVÁ, A. RITTICH, B.

Originální název

Alternative procedure for preparation of Lactobacillus cell lysates

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

The Lactobacillus species play an important role in the food industry.They are also very important for human health due to their positive influence of the gastrointestinal tract functions. Identification of the Lactobacillus species is based on the DNA amplification methods. Molecular methods are essential for correct identification of Lactobacillus at the genus or species levels. Polymerase chain reaction (PCR) is common amplification method used in biological laboratories. PCR-ready DNA must be available to amplification. Enzymes and detergents are used for the crude cell lysates preparation. Enzymes disrupt the cell wall and cell proteins and detergents destroy the plasmatic membrane of the cells. DNA is relased into medium and it is available for purification procedure. Lysozyme, sodium dodecyl sulphate (SDS) and proteinase K are the standard components in the bacterial crude lysates preparations. They are relativelly expensive and sophisticated to storage. The aim of this work was find out if enzymes and detergents presented in the washing powders are capable of cell lysis at adequate quality.

Klíčová slova

crude lysates, Lactobacillus, proteinase K, SDS, DNA

Autoři

FRIČOVÁ, M.; ČUTA, R.; ŠPANOVÁ, A. ; RITTICH, B.

Vydáno

17. 8. 2011

Nakladatel

Česká společnost chemická

Místo

Praha

ISSN

0009-2770

Periodikum

Chemické listy

Ročník

105

Číslo

18

Stát

Česká republika

Strany od

s1011

Strany do

s1011

Strany počet

1