Publication detail

Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis

MASAŘÍK, M. GUMULEC, J. SZTALMACHOVÁ, M. HLAVNA, M. BABULA, P. KŘÍŽKOVÁ, S. RYVOLOVÁ, M. JURAJDA, M. SOCHOR, J. ADAM, V. KIZEK, R.

Original Title

Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis

Type

journal article in Web of Science

Language

English

Original Abstract

Prostate cancer with altered zinc(II) cell metabolism is the second most frequently diagnosed cancer in developed countries. The aim of this article was to optimize immunoseparation protocol based on paramagnetic beads conjugated with protein G for the isolation of metallothionein. We observed strong correlation in all experimental groups and all lysate types (r>0.83 at po0.041) between metallothionein concentration related to viability and concentration of copper(II) ions and cadmium(II) ions in medium.

Keywords

Immunoprecipitation; Metallothionein; Microparticles; Microseparation; Prostate cancer

Authors

MASAŘÍK, M.; GUMULEC, J.; SZTALMACHOVÁ, M.; HLAVNA, M.; BABULA, P.; KŘÍŽKOVÁ, S.; RYVOLOVÁ, M.; JURAJDA, M.; SOCHOR, J.; ADAM, V.; KIZEK, R.

RIV year

2011

Released

21. 11. 2011

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

32

Number

24

State

Federal Republic of Germany

Pages from

3576

Pages to

3588

Pages count

13

BibTex

@article{BUT75925,
  author="Michal {Masařík} and Jaromír {Gumulec} and Markéta {Sztalmachová} and Marian {Hlavna} and Petr {Babula} and Soňa {Křížková} and Markéta {Vaculovičová} and Michal {Jurajda} and Jiří {Sochor} and Vojtěch {Adam} and René {Kizek}",
  title="Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis",
  journal="Electrophoresis",
  year="2011",
  volume="32",
  number="24",
  pages="3576--3588",
  doi="10.1002/elps.201100301",
  issn="0173-0835"
}