Detail publikace

Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis

MASAŘÍK, M. GUMULEC, J. SZTALMACHOVÁ, M. HLAVNA, M. BABULA, P. KŘÍŽKOVÁ, S. RYVOLOVÁ, M. JURAJDA, M. SOCHOR, J. ADAM, V. KIZEK, R.

Originální název

Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Prostate cancer with altered zinc(II) cell metabolism is the second most frequently diagnosed cancer in developed countries. The aim of this article was to optimize immunoseparation protocol based on paramagnetic beads conjugated with protein G for the isolation of metallothionein. We observed strong correlation in all experimental groups and all lysate types (r>0.83 at po0.041) between metallothionein concentration related to viability and concentration of copper(II) ions and cadmium(II) ions in medium.

Klíčová slova

Immunoprecipitation; Metallothionein; Microparticles; Microseparation; Prostate cancer

Autoři

MASAŘÍK, M.; GUMULEC, J.; SZTALMACHOVÁ, M.; HLAVNA, M.; BABULA, P.; KŘÍŽKOVÁ, S.; RYVOLOVÁ, M.; JURAJDA, M.; SOCHOR, J.; ADAM, V.; KIZEK, R.

Rok RIV

2011

Vydáno

21. 11. 2011

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

32

Číslo

24

Stát

Spolková republika Německo

Strany od

3576

Strany do

3588

Strany počet

13

BibTex

@article{BUT75925,
  author="Michal {Masařík} and Jaromír {Gumulec} and Markéta {Sztalmachová} and Marian {Hlavna} and Petr {Babula} and Soňa {Křížková} and Markéta {Vaculovičová} and Michal {Jurajda} and Jiří {Sochor} and Vojtěch {Adam} and René {Kizek}",
  title="Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis",
  journal="Electrophoresis",
  year="2011",
  volume="32",
  number="24",
  pages="3576--3588",
  doi="10.1002/elps.201100301",
  issn="0173-0835"
}