Publication detail

PCR-fingerprrinting as a methods for identification of Saccharomyces yeasts isolated from various Moravian musts

VRÁNOVÁ, D. VADKERTIOVÁ, R. ŠURANSKÁ, H.

Original Title

PCR-fingerprrinting as a methods for identification of Saccharomyces yeasts isolated from various Moravian musts

English Title

PCR-fingerprrinting as a methods for identification of Saccharomyces yeasts isolated from various Moravian musts

Type

abstract

Language

Czech

Original Abstract

We identified and verified of the genus yeasts Hansenispora, Pichia, Saccharomyces a Rhodotorula which participated in fermentation process during the production of the white wine from Moravia wine region of Velké Pavlovice. We found of differences between identification of type yeasts (CCY) by conventional methods and molecular techniques too. For species identification of yeasts, amplification products were digested with restriction enzymes HpaII, MseI, AluI, HaeIII, HinfI, TaqI and CfoI . The profiles of restriction analysis were analysis by BioNumerics programme and dendrogram based on clustering analysis of the Jaccars similarity coefficient was constructed.

English abstract

We identified and verified of the genus yeasts Hansenispora, Pichia, Saccharomyces a Rhodotorula which participated in fermentation process during the production of the white wine from Moravia wine region of Velké Pavlovice. We found of differences between identification of type yeasts (CCY) by conventional methods and molecular techniques too. For species identification of yeasts, amplification products were digested with restriction enzymes HpaII, MseI, AluI, HaeIII, HinfI, TaqI and CfoI . The profiles of restriction analysis were analysis by BioNumerics programme and dendrogram based on clustering analysis of the Jaccars similarity coefficient was constructed.

Keywords

YEASTS, WINE, pcr

Key words in English

YEASTS, WINE, pcr

Authors

VRÁNOVÁ, D.; VADKERTIOVÁ, R.; ŠURANSKÁ, H.

RIV year

2011

Released

3. 5. 2011

Location

sk

ISBN

1336-4839

Periodical

34th Annual Conference on Yeasts, Book of abstracts

Year of study

39

Number

S

State

Slovak Republic

Pages from

113

Pages to

113

Pages count

1

BibTex

@misc{BUT90512,
  author="Dana {Vránová} and Renata {Vadkertiová} and Hana {Šuranská}",
  title="PCR-fingerprrinting as a methods for identification of Saccharomyces yeasts isolated from various Moravian musts",
  year="2011",
  journal="34th Annual Conference on Yeasts, Book of abstracts",
  volume="39",
  number="S",
  pages="113--113",
  address="sk",
  issn="1336-4839",
  note="abstract"
}