Publication detail

Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration

HRSTKA, M. URBAN, O. BABÁK, L.

Original Title

Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration

Type

journal article - other

Language

English

Original Abstract

We studied Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) content and activity in juvenile tree species – broadleaved Fagus sylvatica L. and coniferous Picea abies (L.) Karsten exposed for three growing seasons to ambient (A = 385 mol mol -1) and elevated (E = 700 mol mol-1) CO2 concentrations. Rubisco content was determined by the SDS-PAGE method, Rubisco activity was assayed spectrophotometrically. The highest content of Rubisco enzyme in F. sylvatica was measured immediately after full leaf development followed by a gradual decrease throughout the growing season. By contrast, Rubisco content in P. abies increased markedly during the growing season. In both tree species, down-regulation of Rubisco content in trees cultivated under elevated CO2 concentration was observed. Rubisco activity was stimulated in F. sylvatica by E treatment but not in P. abies. Because no significant differences were found in Rubisco activation state between A and E, we assume that stimulation of Rubisco activity in E is not a consequence of higher carbamylation but could be caused by the release of inhibitors from active sites of Rubisco under elevated CO2.

Keywords

Rubisco content, Rubisco activity, seasonal changes, elevated CO2 concentrations, Fagus sylvatica, Picea abies

Authors

HRSTKA, M.; URBAN, O.; BABÁK, L.

RIV year

2012

Released

11. 5. 2012

Publisher

Institute of Chemistry, Slovak Academy of Sciences

ISBN

0366-6352

Periodical

Chemical Papers

Year of study

66

Number

9

State

Slovak Republic

Pages from

836

Pages to

841

Pages count

6

BibTex

@article{BUT92921,
  author="Miroslav {Hrstka} and Otmar {Urban} and Libor {Babák}",
  title="Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration",
  journal="Chemical Papers",
  year="2012",
  volume="66",
  number="9",
  pages="836--841",
  issn="0366-6352"
}