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HRSTKA, M. URBAN, O. BABÁK, L.
Originální název
Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
We studied Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) content and activity in juvenile tree species – broadleaved Fagus sylvatica L. and coniferous Picea abies (L.) Karsten exposed for three growing seasons to ambient (A = 385 mol mol -1) and elevated (E = 700 mol mol-1) CO2 concentrations. Rubisco content was determined by the SDS-PAGE method, Rubisco activity was assayed spectrophotometrically. The highest content of Rubisco enzyme in F. sylvatica was measured immediately after full leaf development followed by a gradual decrease throughout the growing season. By contrast, Rubisco content in P. abies increased markedly during the growing season. In both tree species, down-regulation of Rubisco content in trees cultivated under elevated CO2 concentration was observed. Rubisco activity was stimulated in F. sylvatica by E treatment but not in P. abies. Because no significant differences were found in Rubisco activation state between A and E, we assume that stimulation of Rubisco activity in E is not a consequence of higher carbamylation but could be caused by the release of inhibitors from active sites of Rubisco under elevated CO2.
Klíčová slova
Rubisco content, Rubisco activity, seasonal changes, elevated CO2 concentrations, Fagus sylvatica, Picea abies
Autoři
HRSTKA, M.; URBAN, O.; BABÁK, L.
Rok RIV
2012
Vydáno
11. 5. 2012
Nakladatel
Institute of Chemistry, Slovak Academy of Sciences
ISSN
0366-6352
Periodikum
Chemical Papers
Ročník
66
Číslo
9
Stát
Slovenská republika
Strany od
836
Strany do
841
Strany počet
6
BibTex
@article{BUT92921, author="Miroslav {Hrstka} and Otmar {Urban} and Libor {Babák}", title="Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration", journal="Chemical Papers", year="2012", volume="66", number="9", pages="836--841", issn="0366-6352" }