Publication detail

Analysis of covalent ellipticine- and doxorubicin-derived adducts in DNA of neuroblastoma cells by the 32P-postlabeling technique

STIBOROVÁ, M. POLJAKOVÁ, J. ECKSCHLAGER, T. KIZEK, R. FREI, E.

Original Title

Analysis of covalent ellipticine- and doxorubicin-derived adducts in DNA of neuroblastoma cells by the 32P-postlabeling technique

Type

journal article in Web of Science

Language

English

Original Abstract

The results presented in this paper are the first to demonstrate that in contrast to covalent DNA adducts formed by ellipticine, the adducts generated by formaldehyde-mediated covalent binding of doxorubicin to DNA are not detectable by the 32P-postlabeling assay. No DNA adducts were, detectable either in vitro, in incubations of DNA with doxorubicin or in DNA of neuroblastoma cells treated with this drug. The results also suggest that covalent binding of ellipticine to DNA of UKF-NB-3 and UKF-NB-4 neuroblastoma cell lines is the predominant mechanism responsible for the cytotoxicity of this drug. To understand the mechanisms of doxorubicin anticancer effects on neuroblastoma cells, development of novel methods for identifying covalent doxorubicin-derived DNA adducts is the major challenge for further research

Keywords

ellipticine, doxorubicin, neuroblastoma, DNA adducts, cancer

Authors

STIBOROVÁ, M.; POLJAKOVÁ, J.; ECKSCHLAGER, T.; KIZEK, R.; FREI, E.

RIV year

2012

Released

1. 6. 2012

ISBN

1213-8118

Periodical

Biomedical Papers

Year of study

156

Number

2

State

Czech Republic

Pages from

115

Pages to

121

Pages count

7

BibTex

@article{BUT95224,
  author="Marie {Stiborová} and Jitka {Poljaková} and Tomáš {Eckschlager} and René {Kizek} and Eva {Frei}",
  title="Analysis of covalent ellipticine- and doxorubicin-derived adducts in DNA of neuroblastoma cells by the 32P-postlabeling technique",
  journal="Biomedical Papers",
  year="2012",
  volume="156",
  number="2",
  pages="115--121",
  doi="10.5507/bp.2012.043",
  issn="1213-8118"
}