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STIBOROVÁ, M. POLJAKOVÁ, J. ECKSCHLAGER, T. KIZEK, R. FREI, E.
Originální název
Analysis of covalent ellipticine- and doxorubicin-derived adducts in DNA of neuroblastoma cells by the 32P-postlabeling technique
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
The results presented in this paper are the first to demonstrate that in contrast to covalent DNA adducts formed by ellipticine, the adducts generated by formaldehyde-mediated covalent binding of doxorubicin to DNA are not detectable by the 32P-postlabeling assay. No DNA adducts were, detectable either in vitro, in incubations of DNA with doxorubicin or in DNA of neuroblastoma cells treated with this drug. The results also suggest that covalent binding of ellipticine to DNA of UKF-NB-3 and UKF-NB-4 neuroblastoma cell lines is the predominant mechanism responsible for the cytotoxicity of this drug. To understand the mechanisms of doxorubicin anticancer effects on neuroblastoma cells, development of novel methods for identifying covalent doxorubicin-derived DNA adducts is the major challenge for further research
Klíčová slova
ellipticine, doxorubicin, neuroblastoma, DNA adducts, cancer
Autoři
STIBOROVÁ, M.; POLJAKOVÁ, J.; ECKSCHLAGER, T.; KIZEK, R.; FREI, E.
Rok RIV
2012
Vydáno
1. 6. 2012
ISSN
1213-8118
Periodikum
Biomedical Papers
Ročník
156
Číslo
2
Stát
Česká republika
Strany od
115
Strany do
121
Strany počet
7
BibTex
@article{BUT95224, author="Marie {Stiborová} and Jitka {Poljaková} and Tomáš {Eckschlager} and René {Kizek} and Eva {Frei}", title="Analysis of covalent ellipticine- and doxorubicin-derived adducts in DNA of neuroblastoma cells by the 32P-postlabeling technique", journal="Biomedical Papers", year="2012", volume="156", number="2", pages="115--121", doi="10.5507/bp.2012.043", issn="1213-8118" }