Detail publikace

Analysis of covalent ellipticine- and doxorubicin-derived adducts in DNA of neuroblastoma cells by the 32P-postlabeling technique

STIBOROVÁ, M. POLJAKOVÁ, J. ECKSCHLAGER, T. KIZEK, R. FREI, E.

Originální název

Analysis of covalent ellipticine- and doxorubicin-derived adducts in DNA of neuroblastoma cells by the 32P-postlabeling technique

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The results presented in this paper are the first to demonstrate that in contrast to covalent DNA adducts formed by ellipticine, the adducts generated by formaldehyde-mediated covalent binding of doxorubicin to DNA are not detectable by the 32P-postlabeling assay. No DNA adducts were, detectable either in vitro, in incubations of DNA with doxorubicin or in DNA of neuroblastoma cells treated with this drug. The results also suggest that covalent binding of ellipticine to DNA of UKF-NB-3 and UKF-NB-4 neuroblastoma cell lines is the predominant mechanism responsible for the cytotoxicity of this drug. To understand the mechanisms of doxorubicin anticancer effects on neuroblastoma cells, development of novel methods for identifying covalent doxorubicin-derived DNA adducts is the major challenge for further research

Klíčová slova

ellipticine, doxorubicin, neuroblastoma, DNA adducts, cancer

Autoři

STIBOROVÁ, M.; POLJAKOVÁ, J.; ECKSCHLAGER, T.; KIZEK, R.; FREI, E.

Rok RIV

2012

Vydáno

1. 6. 2012

ISSN

1213-8118

Periodikum

Biomedical Papers

Ročník

156

Číslo

2

Stát

Česká republika

Strany od

115

Strany do

121

Strany počet

7

BibTex

@article{BUT95224,
  author="Marie {Stiborová} and Jitka {Poljaková} and Tomáš {Eckschlager} and René {Kizek} and Eva {Frei}",
  title="Analysis of covalent ellipticine- and doxorubicin-derived adducts in DNA of neuroblastoma cells by the 32P-postlabeling technique",
  journal="Biomedical Papers",
  year="2012",
  volume="156",
  number="2",
  pages="115--121",
  doi="10.5507/bp.2012.043",
  issn="1213-8118"
}