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ŠIMŠÍKOVÁ, M. ANTALÍK, M. KAŇUCHOVÁ, M. ŠKVARLA, J.
Original Title
Cytochrome c conjugated to ZnO–MAA nanoparticles: The study of interaction and influence on protein structure
Type
journal article - other
Language
English
Original Abstract
Nanoparticle–protein conjugates have potential for numerous applications due to the combination of theproperties of both components. In this paper we studied the conjugation of horse heart cytochrome c withZnO nanoparticles modified by mercaptoacetic acid (MAA) which may be a material with great potentialin anticancer therapy as a consequence of synergic effect of both components. Cyt c adsorption to theZnO–MAA NPs surface was studied by UV–vis spectroscopy and by a dynamic light scattering in variouspH. The results indicate that the optimal pH for the association of protein with modified nanoparticles isin range 5.8–8.5 where 90–96% of cytochrome c was assembled on ZnO–MAA nanoparticles.The interaction of proteins with nanoparticles often results in denaturation or loss of protein func-tion. Our observations from UV–vis spectroscopy and circular dichroism performed preserved proteinstructure after the interaction with modified nanoparticles
Keywords
ZnO nanoparticles; Mercaptoacetic acid; Cytochrome c; Protein interaction
Authors
ŠIMŠÍKOVÁ, M.; ANTALÍK, M.; KAŇUCHOVÁ, M.; ŠKVARLA, J.
RIV year
2013
Released
1. 8. 2013
ISBN
0141-8130
Periodical
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Year of study
59
Number
1
State
Kingdom of the Netherlands
Pages from
235
Pages to
241
Pages count
7
BibTex
@article{BUT99659, author="Michaela {Šimšíková} and Marián {Antalík} and Mária {Kaňuchová} and Jiří {Škvarla}", title="Cytochrome c conjugated to ZnO–MAA nanoparticles: The study of interaction and influence on protein structure", journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES", year="2013", volume="59", number="1", pages="235--241", issn="0141-8130" }