Detail publikačního výsledku

Cytochrome c conjugated to ZnO–MAA nanoparticles: The study of interaction and influence on protein structure

ŠIMŠÍKOVÁ, M.; ANTALÍK, M.; KAŇUCHOVÁ, M.; ŠKVARLA, J.

Originální název

Cytochrome c conjugated to ZnO–MAA nanoparticles: The study of interaction and influence on protein structure

Anglický název

Cytochrome c conjugated to ZnO–MAA nanoparticles: The study of interaction and influence on protein structure

Druh

Článek recenzovaný mimo WoS a Scopus

Originální abstrakt

Nanoparticle–protein conjugates have potential for numerous applications due to the combination of theproperties of both components. In this paper we studied the conjugation of horse heart cytochrome c withZnO nanoparticles modified by mercaptoacetic acid (MAA) which may be a material with great potentialin anticancer therapy as a consequence of synergic effect of both components. Cyt c adsorption to theZnO–MAA NPs surface was studied by UV–vis spectroscopy and by a dynamic light scattering in variouspH. The results indicate that the optimal pH for the association of protein with modified nanoparticles isin range 5.8–8.5 where 90–96% of cytochrome c was assembled on ZnO–MAA nanoparticles.The interaction of proteins with nanoparticles often results in denaturation or loss of protein func-tion. Our observations from UV–vis spectroscopy and circular dichroism performed preserved proteinstructure after the interaction with modified nanoparticles

Anglický abstrakt

Nanoparticle–protein conjugates have potential for numerous applications due to the combination of theproperties of both components. In this paper we studied the conjugation of horse heart cytochrome c withZnO nanoparticles modified by mercaptoacetic acid (MAA) which may be a material with great potentialin anticancer therapy as a consequence of synergic effect of both components. Cyt c adsorption to theZnO–MAA NPs surface was studied by UV–vis spectroscopy and by a dynamic light scattering in variouspH. The results indicate that the optimal pH for the association of protein with modified nanoparticles isin range 5.8–8.5 where 90–96% of cytochrome c was assembled on ZnO–MAA nanoparticles.The interaction of proteins with nanoparticles often results in denaturation or loss of protein func-tion. Our observations from UV–vis spectroscopy and circular dichroism performed preserved proteinstructure after the interaction with modified nanoparticles

Klíčová slova

ZnO nanoparticles; Mercaptoacetic acid; Cytochrome c; Protein interaction

Klíčová slova v angličtině

ZnO nanoparticles; Mercaptoacetic acid; Cytochrome c; Protein interaction

Autoři

ŠIMŠÍKOVÁ, M.; ANTALÍK, M.; KAŇUCHOVÁ, M.; ŠKVARLA, J.

Rok RIV

2014

Vydáno

01.08.2013

ISSN

0141-8130

Periodikum

International Journal of Biological Macromolecules

Svazek

59

Číslo

1

Stát

Nizozemsko

Strany od

235

Strany do

241

Strany počet

7

BibTex

@article{BUT99659,
  author="Michaela {Šimšíková} and Marián {Antalík} and Mária {Kaňuchová} and Jiří {Škvarla}",
  title="Cytochrome c conjugated to ZnO–MAA nanoparticles: The study of interaction and influence on protein structure",
  journal="International Journal of Biological Macromolecules",
  year="2013",
  volume="59",
  number="1",
  pages="235--241",
  issn="0141-8130"
}