Publication detail

Palm-Sized Device for Point-of-Care Ebola Detection

NEUŽIL P. , AHRBERG C. D., MANZ A.

Original Title

Palm-Sized Device for Point-of-Care Ebola Detection

Type

journal article in Web of Science

Language

English

Original Abstract

We show the utilization of a recently developed cellphone-sized real-time polymerase chain reaction (PCR) device to detect Ebola virus RNA using single-step reverse transcription PCR (RT-PCR). The device was shown to concurrently perform four PCRs, each with a sample volume of 100 nL: one positive control with both Ebola and GAPDH RNA and one negative control. The last two positions were used to measure the GAPDH and the Ebola content of a sample. A comparison of threshold cycles (CT) from the two samples provided relative quantification. The entire process, which consisted of reverse transcription, PCR amplification, and melting curve analysis (MCA), was conducted in less than 37 min. The next step will be integration with a sample preparation unit to form an integrated sample-to-answer system for point-of-care infectious disease diagnostics.

Keywords

REVERSE TRANSCRIPTION-PCR; HEMORRHAGIC-FEVER; VIRUS DISEASE; RAPID DETECTION; CONGO; DIAGNOSIS; OUTBREAK; BLOOD; FIELD; CHIP

Authors

NEUŽIL P. , AHRBERG C. D., MANZ A.

Released

3. 5. 2016

Publisher

American Chemical Society

Location

USA

ISBN

1520-6882

Periodical

Analytical Chemistry

Year of study

88

Number

9

State

United States of America

Pages from

4803

Pages to

4807

Pages count

5

URL

http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b00278

BibTex

@article{BUT128960,
  author="Pavel {Neužil} and Christian D. {Ahrberg} and Andreas {Manz}",
  title="Palm-Sized Device for Point-of-Care Ebola Detection",
  journal="Analytical Chemistry",
  year="2016",
  volume="88",
  number="9",
  pages="4803--4807",
  doi="10.1021/acs.analchem.6b00278",
  issn="1520-6882",
  url="http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b00278"
}