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MELČOVÁ, V. KROBOT, Š. ŠINDELÁŘ, J. ŠEBOVÁ, E. KLUSÁČEK RAMPICHOVÁ, M. PŘIKRYL, R.
Original Title
The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces
Type
journal article in Web of Science
Language
English
Original Abstract
The aim of this work is to explore the effect of surface characteristics of the 3D printed scaffolds on their suitability for Saos-2 cells growth. Two promising materials for bone tissue engineering were examined, both containing poly(3-hydroxybutyrate)/poly(d,l-lactide) plasticized blend and one of them filled with bioactive tricalcium phosphate. The surface free energy (SFE) of samples was determined by contact angle measurement with four liquids. For non-filled sample the water contact angle (WCA) was 74° and its SFE was 40 mN∙m−1. The composite sample exhibited substantially decreased WCA, 61°. Moreover, the addition of the tricalcium phosphate caused doubling of the polar component of the SFE which increased by a total of 13%. 3D printed surfaces prepared by fused deposition modeling method showed a profound increase of WCA due to the increase in their surface roughness which was analyzed by confocal microscopy. The change in wetting properties strongly affected the behavior of Saos-2 cells on printed surfaces. The number of cells as measured by DNA quantification linearly decreased with increasing surface WCA. The highest number of cells was observed on the pressed samples and 3D printed surfaces made of simple lines and a grid with 50 μm gap between lines. The favored surfaces exhibit WCA under 80° which is important information for the future design of the scaffolds.
Keywords
Poly(3-hydroxybutyrate); polylactide; 3D printing; water contact angle; surface energy; surface roughness
Authors
MELČOVÁ, V.; KROBOT, Š.; ŠINDELÁŘ, J.; ŠEBOVÁ, E.; KLUSÁČEK RAMPICHOVÁ, M.; PŘIKRYL, R.
Released
1. 8. 2024
Publisher
Elsevier
ISBN
2666-8459
Periodical
Results in Surfaces and Interfaces
Year of study
16
Number
August
State
Kingdom of the Netherlands
Pages from
1
Pages to
18
Pages count
URL
https://www.sciencedirect.com/science/article/pii/S2666845924000916?via%3Dihub
BibTex
@article{BUT189307, author="Veronika {Melčová} and Štěpán {Krobot} and Jan {Šindelář} and Eva {Šebová} and Michala {Klusáček Rampichová} and Radek {Přikryl}", title="The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces", journal="Results in Surfaces and Interfaces", year="2024", volume="16", number="August", pages="1--18", doi="10.1016/j.rsurfi.2024.100271", issn="2666-8459", url="https://www.sciencedirect.com/science/article/pii/S2666845924000916?via%3Dihub" }