Publication detail

Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector

ADAM, V. ZÍTKA, O. DOLEŽAL, P. ZEMAN, L. HORNA, A. HUBÁLEK, J. ŠÍLENÝ, J. KŘÍŽKOVÁ, S. TRNKOVÁ, L. KIZEK, R.

Original Title

Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector

Type

journal article - other

Language

English

Original Abstract

Lactoferrin is a multifunctional protein with antimicrobial activity and others to health beneficial properties. The main aim of this work was to propose easy to use technique for lactoferrin isolation from cow colostrum samples. Primarily we utilized sodium dodecyl sulphate-polyacrylamide gel electrophoresis for isolation of lactoferrin from the real samples. Moreover we tested automated microfluidic Experion electrophoresis system to isolate lactoferrin from the collostrum sample. The well developed signal of lactoferrin was determined with detection limit (3 S/N) of 20 ng/ml. In spite of the fact that Experion is faster than SDS-PAGE both separation techniques cannot be used in routine analysis. Therefore we have tested third separation technique, ion exchange chromatography, using monolithic column coupled with UV-VIS detector (LCUV-VIS). We optimized wave length (280 nm), ionic strength of the elution solution (1.5 M NaCl) and flow rate of the retention and elution solutions (0.25 ml/min and 0.75 ml/min. respectively).

Keywords

Lactoferrin; Milk Protein; Linear Sweep Voltammetry; Screen-Printed Carbon Electrode; Electrochemistry; Liquid Chromatography; Monolithic Column; UV-VIS Spectrometry

Authors

ADAM, V.; ZÍTKA, O.; DOLEŽAL, P.; ZEMAN, L.; HORNA, A.; HUBÁLEK, J.; ŠÍLENÝ, J.; KŘÍŽKOVÁ, S.; TRNKOVÁ, L.; KIZEK, R.

RIV year

2008

Released

24. 1. 2008

Publisher

MDPI

Location

Basel

ISBN

1424-8220

Periodical

SENSORS

Year of study

2008

Number

8

State

Swiss Confederation

Pages from

464

Pages to

487

Pages count

23

URL

http://www.mdpi.com/1424-8220/8/1/464

Full text in the Digital Library

http://hdl.handle.net/11012/70078

BibTex

@article{BUT49158,
  author="ADAM, V. and ZÍTKA, O. and DOLEŽAL, P. and ZEMAN, L. and HORNA, A. and HUBÁLEK, J. and ŠÍLENÝ, J. and KŘÍŽKOVÁ, S. and TRNKOVÁ, L. and KIZEK, R.",
  title="Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector",
  journal="SENSORS",
  year="2008",
  volume="2008",
  number="8",
  pages="464--487",
  doi="10.3390/s8010464",
  issn="1424-8220",
  url="http://www.mdpi.com/1424-8220/8/1/464"
}