Course detail

Bioengineering II

FCH-DCO_BI IIAcad. year: 2011/2012

Course on Bioengineering II is based on the knowledge of Chemical Engineering fundamentals and Bioengineering I and is oriented to engineering and biological principles of processes. Core of the course are unit operations of biotechnological process. It consists in following teaching units: Flow characteristics in bioreactors. Mixing, aeration and oxygen transfer in bioreactors. Industrial bioreactors. Construction of bioreactors, construction material. Auxiliary equipment. Media transport. Process monitoring and control. Aseptic bioreactors. Physiological state of micro-organisms, Cell transport in biological systems. Kinetics of enzyme reactions. Stoichiometry of microbial growth. Kinetics of microbial growth and metabolite production. Mathematical model sof growth of microorganisms and produkt formation (+ survey about optimization methods. Simulation language PSI). Media, their preparation and sterilation. Air sterilation. Fundamental cultivation techniques: batch, fed-batch, continuous and special techniques of cultivations (surface cultivation, cultivation on the interface between hydrophilic and hydrophobic phases, cultivation of plant and animal cells, production by means of immobilized cells). Separation techniques for product isolation and choice of separation method: Membrane separations. Electro-separation processes. Separations based on differences in densities. Chromatographic methods and ion exchange. Adsorption. Extraction. Evaporation. Distillation, Disintegration. Methods for isolation of proteins (incl. enzymes).

Language of instruction

Czech

Mode of study

Not applicable.

Learning outcomes of the course unit

Students receive during the course knowledge about basic unit operations of biotechnological process and their solution. Systematically will be stressed difference in solution with respect to special demands of biological . After passing this course students should be able to design simple bioengineering solution from cultivation side to isolation of products. Economic aspects of industrial production are to be considered. At this goal is also aiming mathematical modeling of biotechnological processes.

Prerequisites

1 Structure and functions of microorganisms
2 Mass and energy balances, unit operations of chemical processes
3 Solution of ordinary differential equations, basic knowledge from analytical geometry, statistics and numerical mathematics.
4 Kinetics of chemical reactions and solution of simple kinetic equations

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Assesment methods and criteria linked to learning outcomes


The scope of the course is determined by lectures/ seminars. Evaluation of the course is complex. In case of a small number of students it is possible to organize the course by means of distance learning. With few obligatory consultations.

Course curriculum

Winter term

1 Flow characteristics in bioreactors. Mixing, aeration and oxygen transfer in bioreactors.
2 Industrial bioreactors. Construction of bioreactors, construction material. Auxiliary equipment. Media transport. Aseptic bioreactors.
3 Measurement, regulation and control of biotechnological processes.
4 Physiological state of micro-organisms, Cell transport in biological systems.
5 Kinetics of enzyme reactions.
6 Stoichiometry of microbial growth.
7 Kinetics of microbial growth and metabolite production.
8 Mathematical models of growth of microorganisms and product formation.
9 Survey on optimization methods.
10 Simulation language PSI.
11 Media, their preparation and sterilation. Air sterilation.
12 Fundamental cultivation techniques: batch, fed-batch, continuous and special techniques of cultivations (surface cultivation, cultivation on the interface between hydrophilic and hydrophobic phases, cultivation of plant and animal cells, production by means of immobilized cells).

Summer term
1 Separation techniques for product isolation and choice of separation method.
2 Membrane separations. Membranes. Microfiltration, ultrafiltration, nanofiltration, reverse osmosis.
3 Membrane separations. Membrane modules, arrangement of a membrane separation process. Electrodialysis. Pervaporation and permaeation of gases. Pervaporation. Membrane distillation. Dialysis.
4 Separations based on differences in densities.
5 Chromatographic methods and ion exchange.
6 Adsorption.
7 Extraction.
8 Evaporation.
9 Distillation,
10 Disintegration.
11 Methods for isolation of proteins (incl. enzymes).


Work placements

Not applicable.

Aims

Aim of the course is to prepare students not only for application of bioengineering in from laboratory to large scale conditions but also to apply bioengineering methods and principles in solution of technological tasks and in solution of dissertation thesis. Extremely important is to understand sequence of operations of microbe, plant and animal cell cultivation and to balance each step of the process. Formulation of mathematical model of corresponding structure can help in optimization of cultivation conditions of cell cultivation and in isolation of microbial products.

Specification of controlled education, way of implementation and compensation for absences

Knowledge will be checked not only by oral exam but also elaborating and oral presentation of technological-engineering project.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

F.Kaštánek: Bioinženýrství, 2001 (CS)
M.Rychtera, J.Páca: Bioinženýrství kvasných procesů, 1985 (CS)

Recommended reading

H.J.Rehm, G.Reed: Biiotechnology, Vol.3 (CS)
H.J.Rehm, G.Reed: Biotechnology, Vol.4 (CS)
J.E.Baily, D.F.Ollis:Biochemical Engineering Fundamentals, 1986 (CS)
M.Moo-Young:Comprehensive Biotechnology, Vol.2, 1985 (CS)

Classification of course in study plans

  • Programme DPCP_CHM Doctoral

    branch DPCO_CHM , 1 year of study, winter semester, compulsory-optional

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1 year of study, winter semester, compulsory-optional

  • Programme DPCP_CHM Doctoral

    branch DPCO_CHM , 2 year of study, winter semester, compulsory-optional

  • Programme DKCP_CHM Doctoral

    branch DKCO_CHM , 1 year of study, winter semester, compulsory-optional
    branch DKCO_CHM , 2 year of study, winter semester, compulsory-optional

Type of course unit

 

Guided consultation in combined form of studies

0 hod., optionally

Teacher / Lecturer